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TitleZymomonas mobilis: a promising microorganism for prebiotic production
Author(s)Braga, Adelaide
Gomes, Daniela
Rainha, João
Amorim, Cláudia Catarina Oliveira
Cardoso, B.
Gudiña, Eduardo José
Silvério, Sara Isabel Cruz
Rodrigues, Joana Lúcia Lima Correia
Rodrigues, L. R.
Issue date23-Nov-2021
CitationBraga, Adelaide; Gomes, Daniela; Amorim, Cláudia; Cardoso, B.; Gudiña, Eduardo J.; Silvério, Sara C.; Rodrigues, Joana L.; Rodrigues, Lígia R., Zymomonas mobilis: a promising microorganism for prebiotic production. Microbiotec 21 - Congress of Microbiology and Biotechnology (Abstracts Book). No. O.5, UNL Online, Nov 23-26, 121, 2021.
Abstract(s)Zymomonas mobilis (ZM) is an ethanologenic bacterium with outstanding characteristics which makes it an interesting chassis for the biotechnological production of prebiotics. Fructooligosaccharides (FOS) are promising prebiotics in the increasing market of functional food. In this work, a Box-Behnken design approach was used to optimize the medium composition and maximize the FOS content. Under optimal conditions, 45.3 g/L of FOS were obtained. Sucrose was the most significant variable; thus, its concentration was further increased to 350 g/L leading to a 1.13-fold enhance in FOS titer. Afterwards, a scale-up to bioreactor was performed resulting in a high yield, content and productivity of FOS (58 %, 156.5 g/L 4.8 g/L h). Furthermore, 45 g/L of sorbitol and 8 g/L of levan were also produced. After purification of the FOS mixture through an activated charcoal column, an in vitro model using human fecal inoculum was used to assess its prebiotic potential. The results suggest that the produced prebiotic mixture has potential to be used to improve the human health. The present work describes for the first time the production of a prebiotic mixture with ZM ZM4 in an in vivo single-step approach that has potential to be commercialized as functional food ingredient.
TypeOral presentation
Publisher version
AccessOpen access
Appears in Collections:CEB - Comunicações Orais / Oral Communications

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