Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/74947

TitleHelicobacter pylori prophages: screening, detection, induction and potential therapeutic use
Author(s)Ferreira, Rute Vanessa Novais
Sousa, Cláudia Sofia Cunha
Presa, Eva
Pires, Diana Priscila Penso
Oleastro, Mónica
Azeredo, Joana
Figueiredo, Céu
Melo, Luís Daniel Rodrigues
Issue date23-Nov-2021
PublisherUniversidade Nova de Lisboa (UNL)
CitationFerreira, R.; Sousa, Cláudia; Presa, Eva; Pires, Diana P.; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel Rodrigues, Helicobacter pylori prophages: screening, detection, induction and potential therapeutic use. Microbiotec 21 - Congress of Microbiology and Biotechnology (Abstracts Book). No. O.432, UNL Online, Nov 23-26, 205-206, 2021.
Abstract(s)Helicobacter pylori is a microaerophilic bacterium that chronically infects the human gastric mucosa. Infections caused by this pathogen are difficult to treat, mainly due to the increased resistance of this species to conventional antibiotics. Therefore, it is important to develop antibiotic alternative or complementary approaches to tackle H. pylori infections. Bacterio(phages) have proven to be efficient antibacterial agents, however it is very difficult to isolate strictly lytic phages infecting H. pylori. Nevertheless, this bacterial species presents prophages in their genomes and although strictly lytic phages have been consensually preferred for phage therapy purposes, temperate prophages holds a great but an exploited potential. In the present work, we developed a new PCR-based screening method to detect the presence of prophages genes in a set of H. pylori Portuguese clinical strains. The genomes of selected strains were then sequenced using a combined Illumina platform and MinION nanopore-based sequencing strategy. Prophages content was then analysed using the PHASTER tool. After sequencing analysis, UV light was used to induce phages, from which one was further characterized in terms of morphology, host range, stability on an in vitro gastric model, genome analysis and efficacy against a H. pylori culture. The complementarity between Illumina and Nanopore results, allowed us to identify a total of 10 intact, 7 questionable and 47 incomplete prophages on the 14 sequenced strains. One predicted intact prophage was induced successfully, and presents a genome length of 31 162 bp with 37.1 % G+C content. Interestingly, this new podovirus infects five H. pylori strains, and in the gastric in vitro model only a small loss of phage titer was observed in the gastric phase, suggesting that this phage could be adapted to the stomach environment. Farther, this phage demonstrated to be capable of maintaining the H. pylori population at low levels for up to 24 h post-infection with MOIs of 0.01, 0.1 and 1. Overall, a new PCR screening method was developed to detect prophages on H. pylori and positive correlations with sequencing results were observed. Moreover, this new isolated phage seems to have therapeutic potential to treat H. pylori gastric infections.
TypeAbstract
URIhttps://hdl.handle.net/1822/74947
Publisher versionhttps://microbiotec21.organideia.pt/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

Files in This Item:
File Description SizeFormat 
document_55068_1.pdf255,05 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID