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https://hdl.handle.net/1822/75177
Título: | The putative toxin-antitoxin mazEF gene cluster is not involved in cell death in S. epidermidis |
Autor(es): | Gaio, Vânia Silva Cerca, Nuno França, Ângela Maria Oliveira Sousa |
Data: | 23-Nov-2021 |
Citação: | Gaio, Vania; Cerca, Nuno; França, Angela, The putative toxin-antitoxin mazEF gene cluster is not involved in cell death in S. epidermidis. Microbiotec 21 - Congress of Microbiology and Biotechnology (Abstracts Book). No. 140, UNL Online, Nov 23-26, 254, 2021. |
Resumo(s): | Background: Toxin-antitoxin (TA) systems are known to work as a poison-antidote system, where the action of the antitoxin neutralizes the effect of the toxin counterpart. mazEF has been identified as a TA system in several species and is associated with the bacterial stress response. A previous study showed that the formation of viable but non-culturable (VBNC) cells in S. epidermidis biofilms might be linked to the gene cluster mazEF, raising interest in the study of the function of mazEF operon in this species. Objectives: Since mazEF function as a TA system in S. epidermidis has been attributed based uniquely on protein homology with other species, herein we aimed to confirm whether the mazEF operon plays a role as a TA system by constructing a mutant in S. epidermidis 1457 background. Additionally, the effect of mazEF deletion on some virulence factors of this bacteria was also assessed. Results: Interestingly, our results showed that mazEF did not act as a TA system, apparently playing a distinct role in S. epidermidis, as no toxic effect was verified upon expression of mazF, the putative toxin, in mazEF-mutant cells. Moreover, it seems that the mazEF operon has a minor impact on the antimicrobial tolerance of this strain, although we observed a link between mazEF deletion and expression of other known virulence genes. Conclusions and significance: Although an indirect role in virulent gene modulation was found, our data suggest that mazEF is not a TA system and we failed to observe a direct role on virulence. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/75177 |
Versão da editora: | https://microbiotec21.organideia.pt/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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document_55073_1.pdf | 205,62 kB | Adobe PDF | Ver/Abrir |