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https://hdl.handle.net/1822/75282
Título: | Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella |
Autor(es): | Araújo, Daniela Mil-Homens, Dalila Henriques, Mariana Silva, Sónia Carina |
Palavras-chave: | 2′-OMethyl chemical modification antisense oligonucleotides candidiasis Galleria mellonella virulence |
Data: | 8-Mar-2022 |
Editora: | Cell Press |
Revista: | Molecular Therapy - Nucleic Acids |
Citação: | Araújo, Daniela; Mil-Homens, Dalila; Henriques, Mariana; Silva, Sónia Carina, Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella. Molecular Therapy-Nucleic Acids, 27, 517-523, 2022 |
Resumo(s): | EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75282 |
DOI: | 10.1016/j.omtn.2021.12.018 |
ISSN: | 2162-2531 |
Versão da editora: | https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(21)00321-8 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55100_1.pdf | 4,33 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons