Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75314
Título: | Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface |
Autor(es): | Pereira, D. R. Silva-Correia, J. Oliveira, J. M. Reis, R. L. Pandit, A. |
Palavras-chave: | 3D hydrogels Immune regeneration INTERFACE Stem cells Tissue engineering 3D hydrogels stem cells Interface tissue engineering |
Data: | 2022 |
Editora: | Elsevier |
Revista: | Materials Science & Engineering: C |
Citação: | Pereira D. R., Silva-Correia J., Oliveira J. M., Reis R. L., Pandit A. Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface, Materials Science & Engineering C, pp. 112611, doi:10.1016/j.msec.2021.112611, 2021 |
Resumo(s): | The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine. The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function. The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate. Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA). The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel. The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers. Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs. The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining. Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs. A homogeneous distribution was achieved in a successful cellular tissue-to-tissue interface. Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs. The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype. The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu. Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate. Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75314 |
DOI: | 10.1016/j.msec.2021.112611 |
ISSN: | 0928-4931 |
Versão da editora: | https://doi.org/10.1016/j.msec.2021.112611 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20682-pereira-dr-et-al-2021-mat-sci-eng-c.pdf Acesso restrito! | 3,79 MB | Adobe PDF | Ver/Abrir |