Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/75506

TitleNew insights into the acetate uptake transporter (AceTr) family: Unveiling amino acid residues critical for specificity and activity
Author(s)Rendulic, Toni
Alves, João
Azevedo-Silva, João
Soares-Silva, Isabel
Casal, Margarida
KeywordsAto1
Carboxylic acids
Plasma membrane transport
Yeast
Transporter engineering
Issue date2021
PublisherElsevier
JournalComputational and Structural Biotechnology Journal
CitationRendulić, T., Alves, J., Azevedo-Silva, J., Soares-Silva, I., & Casal, M. (2021). New insights into the acetate uptake transporter (AceTr) family: Unveiling amino acid residues critical for specificity and activity. Computational and Structural Biotechnology Journal, 19, 4412-4425. doi: https://doi.org/10.1016/j.csbj.2021.08.002
Abstract(s)Aiming at improving the transport of biotechnologically relevant carboxylic acids in engineered microbial cell factories, the focus of this work was to study plasma membrane transporters belonging to the Acetate Uptake Transporter (AceTr) family. Ato1 and SatP, members of this family from Saccharomyces cerevisiae and Escherichia coli, respectively, are the main acetate transporters in these species. The analysis of conserved amino acid residues within AceTr family members combined with the study of Ato1 3D model based on SatP, was the rationale for selection of site-directed mutagenesis targets. The library of Ato1-GFP mutant alleles was functionally analysed in the S. cerevisiae IMX1000 strain which shows residual growth in all carboxylic acids tested. A gain of function phenotype was found for mutations in the residues F98 and L219 located at the central constrictive site of the pore, enabling cells to grow on lactic and on succinic acid. This phenotype was associated with an increased transport activity for these substrates. A dominant negative acetic acid hypersensitivity was induced in S. cerevisiae cells expressing the E144A mutant, which was associated with an increased acetic acid uptake. By utilizing computer-assisted 3D-modelling tools we highlight structural features that explain the acquired traits found in the analysed Ato1 mutants. Additionally, we achieved the proper expression of the Escherichia coli SatP, a homologue of Ato1, in S. cerevisiae. To our knowledge, this constitutes the first report of a fully functional bacterial plasma membrane transporter protein in yeast cells.
TypeArticle
URIhttps://hdl.handle.net/1822/75506
DOI10.1016/j.csbj.2021.08.002
ISSN2001-0370
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S2001037021003330
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CBMA - Artigos/Papers

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