Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75510
Título: | Protein-based films functionalized with a truncated antimicrobial peptide sequence display broad antimicrobial activity |
Autor(es): | da Costa, André Pereira, Ana M. Sampaio, Paula Rodriguez-Cabello, Jose Carlos Gomes, Andreia C Casal, Margarida Machado, Raul |
Palavras-chave: | Antimicrobial peptides Antimicrobial materials Elastin-like recombinamers Multifunctional materials Skin infections |
Data: | 2021 |
Editora: | American Chemical Society |
Revista: | ACS Biomaterials Science & Engineering |
Resumo(s): | The increasing bacterial resistance to antibiotics is driving strong demand for new antimicrobial biomaterials. This work describes the fabrication of free-standing films exhibiting antimicrobial properties by combining, in the same polypeptide chain, an elastin-like recombinamer comprising 200 repetitions of the pentamer VPAVG (A200) and an 18-amino-acid truncated variant of the antimicrobial peptide BMAP-28, termed BMAP-18. The fusion protein BMAP-18A200 was overexpressed and conveniently purified by a simplified and scalable nonchromato-graphic process. Free-standing films of BMAP-18A200 demonstrated to be stable without requiring cross-linking agents and displayed high antimicrobial activity against skin pathogens including Gram-negative and Gram-positive bacteria as well as unicellular and filamentous fungi. The antimicrobial activity of the films was mediated by direct contact of cells with the film surface, resulting in compromised structural integrity of microbial cells. Furthermore, the BMAP-18A200 films showed no cytotoxicity on normal human cell lines (skin fibroblasts and keratinocytes). All of these results highlight the potential of these biotechnological multifunctional polymers as new drug-free materials to prevent and treat microbial infections. |
Tipo: | Artigo |
Descrição: | The Supporting Information is available free of charge at: https://pubs.acs.org/doi/10.1021/acsbiomaterials.0c01262. |
URI: | https://hdl.handle.net/1822/75510 |
DOI: | 10.1021/acsbiomaterials.0c01262 |
ISSN: | 2373-9878 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acsbiomaterials.0c01262 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
2021-A102. da Costa et al. ACS Biomaterials Science & Engineering.pdf Acesso restrito! | 660,13 kB | Adobe PDF | Ver/Abrir |