Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75594
Título: | Human platelet lysate-loaded Poly(ethylene glycol) hydrogels induce stem cell chemotaxis in vitro |
Autor(es): | Chahal, A. S. Gomez-Florit, Manuel Domingues, Rui Miguel Andrade Gomes, Manuela E. Tiainen, H. |
Palavras-chave: | Bone CHEMOKINES EXPRESSION GROWTH-FACTOR Migration Proteins RECEPTORS Regeneration TISSUE-REPAIR |
Data: | Ago-2021 |
Editora: | American Chemical Society |
Revista: | Biomacromolecules |
Citação: | Chahal, A. S., Gómez-Florit, M., Domingues, R. M., Gomes, M. E., & Tiainen, H. (2021). Human Platelet Lysate-Loaded Poly (ethylene glycol) Hydrogels Induce Stem Cell Chemotaxis In Vitro. Biomacromolecules, 22(8), 3486-3496. |
Resumo(s): | Platelet lysates (PL) contain a selection of proteins and growth factors (GFs) that are known to mediate cell activity. Many of these biomolecules have been identified as chemoattractants with the capacity to induce cell migration. In order to effectively deliver and retain these biomolecules to the site of injury, a scaffold containing PL could be an option. We use poly(ethylene glycol) (PEG) hydrogels consisting of 90 vol % PL to investigate their migratory potential on human mesenchymal stem cells (hMSCs). Cells exposed to these hydrogels were tracked, resulting in cell trajectories and detailed migratory parameters (velocity, Euclidean distance, directness, and forward migration index). Volumetric swelling ratios, hydrogel mechanical properties, and the release kinetics of proteins and GFs from hydrogels were also assessed. Furthermore, hMSC spheroids were encapsulated within the hydrogels to qualitatively assess cell invasion by means of sprouting and disintegration of the spheroid. Cell spheroids encapsulated within the PL-PEG gels exhibited initial outgrowths and eventually colonized the 3D matrix successfully. Results from this study confirmed that hMSCs exhibit directional migration toward the PL-loaded hydrogel with increased velocity and directness, compared to the controls. Overall, the incorporation of PL renders the PEG hydrogel bioactive. This study demonstrates the capacity of PL-loaded hydrogel constructs to attract stem cells for endogenous tissue engineering purposes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75594 |
DOI: | 10.1021/acs.biomac.1c00573 |
ISSN: | 1525-7797 |
Versão da editora: | https://pubs.acs.org/doi/full/10.1021/acs.biomac.1c00573 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20714-acsbiomac1c00573.pdf | 4,6 MB | Adobe PDF | Ver/Abrir |