Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/75652

TitlemazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
Author(s)Gaio, Vânia Silva
Lima, Tânia
Vilanova, Manuel
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
KeywordsSERP1681
SERP1682
antimicrobial tolerance
biofilms
human blood
human plasma
macrophages
dendritic cells
Issue date13-Jan-2022
PublisherFrontiers Media S. A.
JournalFrontiers in Cellular and Infection Microbiology
CitationGaio, Vania; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Angela, mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential. Frontiers in Cellular and Infection Microbiology, 11(803134), 2022
Abstract(s)Staphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to their putative role as a toxinantitoxin system of the mazEF family. Herein, we constructed an S. epidermidis mutant lacking the mazEF genes homologues and determined their role in (i) VBNC state induction during biofilm formation, (ii) antimicrobial susceptibility, (iii) survival in human blood and plasma, and (iv) activation of immune cells. Our results revealed that mazEF homologue did not affect the proportion of VBNC cells in S. epidermidis 1457, refuting the previous hypothesis that mazEF homologue could be linked with the emergence of VBNC cells in S. epidermidis biofilms. Additionally, mazEF homologue did not seem to influence key virulence factors on this strain, since its deletion did not significantly affect the mutant biofilm formation capacity, antimicrobial tolerance or the response by immune cells. Surprisingly, our data suggest that mazEF does not behave as a toxinantitoxin system in S. epidermidis strain 1457, since no decrease in the viability and culturability of bacteria was found when only the mazF toxin homologue was being expressed.
TypeArticle
DescriptionThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.803134/ full#supplementary-material
URIhttps://hdl.handle.net/1822/75652
DOI10.3389/fcimb.2021.803134
ISSN2235-2988
Publisher versionhttps://www.frontiersin.org/journals/cellular-and-infection-microbiology
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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