Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/75810

TitleAccurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency
Author(s)Cerca, Nuno
Lima, Ângela Martins
França, Ângela Maria Oliveira Sousa
KeywordsgDNA extraction efficiency
gDNA qPCR bacterial quantification
Polimicrobial communities
Bacterial vaginosis
Issue dateMar-2022
PublisherElsevier
JournalJournal of Microbiological Methods
CitationCerca, Nuno; Lima, Â.; França, Angela, Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency. Journal of Microbiological Methods, 194(106421), 2022
Abstract(s)qPCR absolute quantification requires the assessment of PCR reaction efficiency. This is achieved by performing serial dilutions of gDNA. Herein, we demonstrate that when quantifying bacterial load in mixed samples, reaction efficiency should not be used as a calibration curve since gDNA isolation efficiency is neglected, significantly impacting quantification.
TypeArticle
URIhttps://hdl.handle.net/1822/75810
DOI10.1016/j.mimet.2022.106421
ISSN0167-7012
Publisher versionhttp://www.journals.elsevier.com/journal-of-microbiological-methods/
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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