1. Universidade do Minho
  2. Escola de Engenharia | School of Engineering
  3. Centro de Engenharia Biológica | Centre of Biological Engineering
  4. CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/75810

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Campo DCValorIdioma
dc.contributor.authorCerca, Nunopor
dc.contributor.authorLima, Ângela Martinspor
dc.contributor.authorFrança, Ângela Maria Oliveira Sousapor
dc.date.accessioned2022-02-07T09:30:27Z-
dc.date.issued2022-03-
dc.identifier.citationCerca, Nuno; Lima, Â.; França, Angela, Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency. Journal of Microbiological Methods, 194(106421), 2022por
dc.identifier.issn0167-7012por
dc.identifier.urihttps://hdl.handle.net/1822/75810-
dc.description.abstractqPCR absolute quantification requires the assessment of PCR reaction efficiency. This is achieved by performing serial dilutions of gDNA. Herein, we demonstrate that when quantifying bacterial load in mixed samples, reaction efficiency should not be used as a calibration curve since gDNA isolation efficiency is neglected, significantly impacting quantification.por
dc.description.sponsorshipFCT -Fundação para a Ciência e a Tecnologia(UIDB/04469/2020)por
dc.language.isoengpor
dc.publisherElsevierpor
dc.relationUIDB/04469/2020por
dc.rightsrestrictedAccesspor
dc.subjectgDNA extraction efficiencypor
dc.subjectgDNA qPCR bacterial quantificationpor
dc.subjectPolimicrobial communitiespor
dc.subjectBacterial vaginosispor
dc.titleAccurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiencypor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.journals.elsevier.com/journal-of-microbiological-methods/por
dc.commentsCEB55248por
oaire.citationIssue106421por
oaire.citationConferencePlaceNetherlands-
oaire.citationVolume194por
dc.date.updated2022-02-05T10:49:57Z-
dc.identifier.doi10.1016/j.mimet.2022.106421por
dc.date.embargo10000-01-01-
dc.identifier.pmid35114291por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersion-
dc.subject.wosScience & Technologypor
sdum.journalJournal of Microbiological Methodspor
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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