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https://hdl.handle.net/1822/75813
Título: | Expression of Yarrowia lipolytica acetyl-CoA carboxylase in Saccharomyces cerevisiae and its effect on in-vivo accumulation of Malonyl-CoA |
Autor(es): | Pereira, Humberto Azevedo, Flávio Domingues, Lucília Johansson, Björn |
Palavras-chave: | Fatty acids Malonyl-CoA Biosensor Saccharomyces cerevisiae ACC1 Yarrowia lipolytica |
Data: | 22-Jan-2022 |
Editora: | Elsevier |
Revista: | Computational and Structural Biotechnology Journal |
Citação: | Pereira, Humberto; Azevedo, Flávio; Domingues, Lucília; Johansson, Björn, Expression of Yarrowia lipolytica acetyl-CoA carboxylase in Saccharomyces cerevisiae and its effect on in-vivo accumulation of Malonyl-CoA. Computational and Structural Biotechnology Journal, 20, 779-787, 2022 |
Resumo(s): | Malonyl-CoA is an energy-rich molecule formed by the ATP-dependent carboxylation of acetyl coenzyme A catalyzed by acetyl-CoA carboxylase. This molecule is an important precursor for many biotechnologically interesting compounds such as flavonoids, polyketides, and fatty acids. The yeast Saccharomyces cerevisiae remains one of the preferred cell factories, but has a limited capacity to produce malonyl-CoA compared to oleaginous organisms. We developed a new S. cerevisiae strain with a conditional allele of ACC1, the essential acetyl-CoA carboxylase (ACC) gene, as a tool to test heterologous genes for complementation. Yarrowia lipolytica is an oleaginous yeast with a higher capacity for lipid production than S. cerevisiae, possibly due to a higher capacity to produce malonyl-CoA. Measuring relative intracellular malonyl-CoA levels with an in-vivo biosensor confirmed that expression of Y. lipolytica ACC in S. cerevisiae leads to a higher accumulation of malonyl-CoA compared with overexpression of the native gene from an otherwise identical vector. The higher accumulation was generally accompanied by a decreased growth rate. Concomitant expression of both the homologous and heterologous ACC1 genes eliminated the growth defect, with a marginal reduction of malonyl-CoA accumulation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75813 |
DOI: | 10.1016/j.csbj.2022.01.020 |
ISSN: | 2001-0370 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S2001037022000277 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55207_1.pdf | 2,18 MB | Adobe PDF | Ver/Abrir |