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https://hdl.handle.net/1822/76486
Título: | Engineering Escherichia coli towards the production of prenylflavonoids |
Autor(es): | Gomes, Daniela Ferreira Castro Rodrigues, Joana Lúcia Lima Correia Rodrigues, L. R. |
Data: | 24-Set-2021 |
Citação: | Gomes, Daniela; Rodrigues, Joana L.; Rodrigues, Lígia R., Engineering Escherichia coli towards the production of prenylflavonoids. 2nd Chemical & Biological Engineering Doctoral Symposium. Braga, Portugal, Sep 22-24, 2021. |
Resumo(s): | Prenylflavonoids are flavonoid-derived compounds with a wide variety of recognized biological activities, namely estrogenic, antioxidant, anti-inflammatory and anticancer. Xanthohumol and 8-prenylnaringenin, which are isolated from Humulus lupulus, have potential to be used as drugs for cancer and menopausal symptoms treatment, respectively. However, these compounds are found at low amounts in nature and their extraction from plants is difficult, expensive and renders impure products. Additionally, these compounds are also hard to chemically synthesize. In addition, these processes are considered not environmentally friendly. For these reasons, the biosynthetic pathway reconstruction in a heterologous host appeared as an alternative method to produce these compounds in higher amounts in a rapid, cheap, and more environmentally friendly way. Herein, we intend to develop an engineered Escherichia coli strain able to produce prenylflavonoids from simple carbon sources such as glucose. The prenylflavonoids biosynthetic pathway in plants starts with the phenylpropanoid pathway that is responsible for the conversion of the aromatic amino acid precursor (tyrosine) to p-coumaric acid in a reaction catalyzed by tyrosine ammonia lyase (TAL). Afterwards, p-coumaric acid is converted to prenylflavonoids through the action of several enzymes. As a starting point for this work, TAL from Rhodotorula glutanis (RgTAL) and TAL from Flavobacterium johnsoniae (FjTAL) were expressed in three different E. coli strains (E. coli BL21, E. coli K12 MG1655 and E. coli M-PAR-121, a tyrosine overproducing strain) to choose the best enzyme and strain to produce p-coumaric acid. The higher production was achieved by the E. coli M-PAR-121 strain expressing FjTAL (2.46 g/L of p-coumaric acid from glucose). This strain and enzyme will be used in the construction of prenylflavonoids biosynthetic pathway. |
Tipo: | Comunicação em painel |
Descrição: | Apresentação efetuada em "2nd Chemical & Biological Engineering Doctoral Symposium", em Braga, 2021 |
URI: | https://hdl.handle.net/1822/76486 |
Versão da editora: | https://www.ceb.uminho.pt/Events/Details/4331 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55211_1.pdf | 988,61 kB | Adobe PDF | Ver/Abrir |