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Título: | Biochemical and molecular characterization of Saccharomyces cerevisiae strains obtained from sugar-cane juice fermentations and their impact in “cachaça” production |
Autor(es): | Oliveira, Valdinéia Aparecida Vicente, Maristela Araújo Fietto, L. G. Castro, I. M. Coutrim, Maurício Xavier Schuller, Dorit Elisabeth Alves, Henrique Casal, Margarida Santos, Juliana de Oliveira Araújo, Leandro Dias Silva, Paulo Henrique Alves da Brandão, R. L. |
Palavras-chave: | Sugar cane Cachaça Saccharomyces cerevisiae Yeast Fermentation Minas Gerais Microsatellites Mitochondrial DNA RFLP mtDNA RFLP Karyotype Trifluoroleucine Cerulenine |
Data: | 2008 |
Editora: | American Society for Microbiology |
Revista: | Applied and Environmental Microbiology |
Citação: | "Applied and Environmental Microbiology". ISSN 0099-2240. 74:3 (2008) 693-701. |
Resumo(s): | Saccharomyces cerevisiae strains from different regions of Minas Gerais, Brazil, were isolated and characterized aiming at the selection of starter yeasts to be used in the production of cachac¸a, the Brazilian sugar cane spirit. The methodology established took into account the screening for biochemical traits desirable in a yeast cachaça producer, such as no H2S production, high tolerance to ethanol and high temperatures, high fermentative capacity, and the abilities to flocculate and to produce mycocins. Furthermore, the yeasts were exposed to drugs such as 5,5_,5_-trifluor-D,L-leucine and cerulenin to isolate those that potentially overproduce higher alcohols and esters. The utilization of a random amplified polymorphic DNA-PCR method with primers based on intron splicing sites flanking regions of the COX1 gene, as well as microsatellite analysis, was not sufficient to achieve good differentiation among selected strains. In contrast, karyotype analysis allowed a clear distinction among all strains. Two selected strains were experimentally evaluated as cachac¸a producers. The results suggest that the selection of strains as fermentation starters requires the combined use of biochemical and molecular criteria to ensure the isolation and identification of strains with potential characteristics to produce cachaça with a higher quality standard. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/7667 |
DOI: | 10.1128/AEM.01729-07 |
ISSN: | 0099-2240 1098-5336 |
Versão da editora: | http://aem.asm.org/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2008_AEM_Cachaça.pdf | Artigo | 485,47 kB | Adobe PDF | Ver/Abrir |