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https://hdl.handle.net/1822/77618
Título: | One-step production of a novel prebiotic mixture using Zymomonas mobilis ZM4 |
Autor(es): | Braga, Adelaide Correia Gomes, Daniela Ferreira Castro Amorim, Cláudia Catarina Oliveira Silvério, Sara Isabel Cruz Alves, Joana I. Rainha, João Cardoso, Beatriz B. Rodrigues, Joana Lúcia Lima Correia Rodrigues, L. R. |
Palavras-chave: | Fructooligosaccharides (FOS) Prebiotic Human fecal inocula In vitro fermentation Zymomonas mobilis |
Data: | Jun-2022 |
Editora: | Elsevier |
Revista: | Biochemical Engineering Journal |
Citação: | Braga, Adelaide; Gomes, Daniela; Amorim, Cláudia; Silvério, Sara C.; Alves, Joana I.; Rainha, João; Cardoso, B.; Rodrigues, Joana L.; Rodrigues, Lígia R., One-step production of a novel prebiotic mixture using Zymomonas mobilis ZM4. Biochemical Engineering Journal, 183(108443), 2022 |
Resumo(s): | Zymomonas mobilis ZM4 presents interesting features for the biotechnological production of prebiotics. In this work, a Box-Behnken design approach was used to optimize the medium composition and maximize the fructooligosaccharides (FOS) production, in a one-step process. Afterwards, the scale-up to bioreactor allowed a high yield, titer and productivity of FOS (0.52 ± 0.05 gFOS gSuc-1, 156.50 ± 0.58gL-1, 4.89 ± 0.88gL-1 h-1). Furthermore, after 72h, 86.69 ± 3.70gL-1 of ethanol, 8.0 ± 0.9gL-1 of levan and 45.62 ± 2.70gL-1 of sorbitol were also produced. After purification (purity degree of 80.9%), an in vitro model using human fecal inoculum was used to assess the prebiotic potential of the FOS-enriched mixture. Herein we describe for the first time a one-step approach to produce a prebiotic mixture with market potential as a functional food ingredient using Z. mobilis ZM4. |
Tipo: | Artigo |
Descrição: | "Available online 29 April 2022" |
URI: | https://hdl.handle.net/1822/77618 |
DOI: | 10.1016/j.bej.2022.108443 |
ISSN: | 1369-703X |
Versão da editora: | http://www.elsevier.com/locate/issn/1369703X |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55519_1.pdf Acesso restrito! | 1,21 MB | Adobe PDF | Ver/Abrir |