Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/77927

TítuloA fibrin coating method of polypropylene meshes enables the adhesion of menstrual blood-derived mesenchymal stromal cells: a new delivery strategy for stem cell-based therapies
Autor(es)Marinaro, Federica
Silva, Joana Maria Marques
Barros, Alexandre António Antunes
Aroso, Ivo Manuel Ascensão
Gómez-Blanco, Juan C.
Jardin, Isaac
Lopez, Jose J.
Pulido, María
Pedro, María Ángeles de
Reis, R. L.
Sánchez-Margallo, Francisco Miguel
Casado, Javier G.
López, Esther
Palavras-chaveSurgical mesh
Fibrin sealant
Coating
Fibrin
Cell therapy
Menstrual blood-derived stromal cell
menstrual blood-derived stromal cells
Data13-Dez-2021
EditoraMultidisciplinary Digital Publishing Institute (MDPI)
RevistaInternational Journal of Molecular Sciences
CitaçãoMarinaro, F.; Silva, J.M.; Barros, A.A.; Aroso, I.M.; Gómez-Blanco, J.C.; Jardin, I.; Lopez, J.J.; Pulido, M.; de Pedro, M.Á.; Reis, R.L.; Sánchez-Margallo, F.M.; Casado, J.G.; López, E. A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies. Int. J. Mol. Sci. 2021, 22, 13385. https://doi.org/10.3390/ijms222413385
Resumo(s)Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (<i>p</i> < 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses.
TipoArtigo
URIhttps://hdl.handle.net/1822/77927
DOI10.3390/ijms222413385
ISSN1422-0067
Versão da editorahttps://www.mdpi.com/1422-0067/22/24/13385
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:BUM - MDPI

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