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https://hdl.handle.net/1822/78692| Title: | Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4 |
| Author(s): | Braga, Adelaide Gomes, Daniela Filipa Correia Costa, João Manuel Rainha Cardoso, Beatriz Amorim, Cláudia Silvério, Sara Isabel Cruz Fernández-Lobato, María Rodrigues, Joana Lúcia Lima Correia Rodrigues, L. R. |
| Keywords: | 6-kestose Fructooligosaccharides (FOS) Levansucrase (sacB) One-step fermentation Schwanniomyces occidentalis (Ffase-Leu196) Zymomonas mobilis |
| Issue date: | 24-Jun-2022 |
| Publisher: | Springer Nature |
| Journal: | Applied Microbiology and Biotechnology |
| Citation: | Braga, A., Gomes, D., Rainha, J. et al. Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4. Appl Microbiol Biotechnol 106, 4617–4626 (2022). https://doi.org/10.1007/s00253-022-12037-3 |
| Abstract(s): | Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated -fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4±1.6 g L1 of FOS, with a productivity of 1.53±0.03 g L1 h1 and a yield of 0.31±0.03 gFOS gsucrose1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. |
| Type: | Article |
| Description: | The online version contains supplementary material available at https://doi.org/10.1007/s00253-022-12037-3 |
| URI: | https://hdl.handle.net/1822/78692 |
| DOI: | 10.1007/s00253-022-12037-3 |
| ISSN: | 0175-7598 |
| Publisher version: | https://link.springer.com/article/10.1007/s00253-022-12037-3 |
| Peer-Reviewed: | yes |
| Access: | Restricted access (UMinho) |
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Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| document_55611_1.pdf Restricted access | 1,11 MB | Adobe PDF | View/Open |
