Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/78692

TitleTailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
Author(s)Braga, Adelaide
Gomes, Daniela Filipa Correia
Costa, João Manuel Rainha
Cardoso, Beatriz
Amorim, Cláudia
Silvério, Sara Isabel Cruz
Fernández-Lobato, María
Rodrigues, Joana Lúcia Lima Correia
Rodrigues, L. R.
Keywords6-kestose
Fructooligosaccharides (FOS)
Levansucrase (sacB)
One-step fermentation
Schwanniomyces occidentalis (Ffase-Leu196)
Zymomonas mobilis
Issue date24-Jun-2022
PublisherSpringer Nature
JournalApplied Microbiology and Biotechnology
CitationBraga, A., Gomes, D., Rainha, J. et al. Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4. Appl Microbiol Biotechnol 106, 4617–4626 (2022). https://doi.org/10.1007/s00253-022-12037-3
Abstract(s)Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated -fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4±1.6 g L1 of FOS, with a productivity of 1.53±0.03 g L1 h1 and a yield of 0.31±0.03 gFOS gsucrose1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process.
TypeArticle
DescriptionThe online version contains supplementary material available at https://doi.org/10.1007/s00253-022-12037-3
URIhttps://hdl.handle.net/1822/78692
DOI10.1007/s00253-022-12037-3
ISSN0175-7598
Publisher versionhttps://link.springer.com/article/10.1007/s00253-022-12037-3
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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