Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78692| Título: | Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4 |
| Autor(es): | Braga, Adelaide Gomes, Daniela Filipa Correia Costa, João Manuel Rainha Cardoso, Beatriz Amorim, Cláudia Silvério, Sara Isabel Cruz Fernández-Lobato, María Rodrigues, Joana Lúcia Lima Correia Rodrigues, L. R. |
| Palavras-chave: | 6-kestose Fructooligosaccharides (FOS) Levansucrase (sacB) One-step fermentation Schwanniomyces occidentalis (Ffase-Leu196) Zymomonas mobilis |
| Data: | 24-Jun-2022 |
| Editora: | Springer Nature |
| Revista: | Applied Microbiology and Biotechnology |
| Citação: | Braga, A., Gomes, D., Rainha, J. et al. Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4. Appl Microbiol Biotechnol 106, 4617–4626 (2022). https://doi.org/10.1007/s00253-022-12037-3 |
| Resumo(s): | Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated -fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4±1.6 g L1 of FOS, with a productivity of 1.53±0.03 g L1 h1 and a yield of 0.31±0.03 gFOS gsucrose1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. |
| Tipo: | Artigo |
| Descrição: | The online version contains supplementary material available at https://doi.org/10.1007/s00253-022-12037-3 |
| URI: | https://hdl.handle.net/1822/78692 |
| DOI: | 10.1007/s00253-022-12037-3 |
| ISSN: | 0175-7598 |
| Versão da editora: | https://link.springer.com/article/10.1007/s00253-022-12037-3 |
| Arbitragem científica: | yes |
| Acesso: | Acesso restrito UMinho |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| document_55611_1.pdf Acesso restrito! | 1,11 MB | Adobe PDF | Ver/Abrir |
