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TitleTailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
Author(s)Braga, Adelaide
Gomes, Daniela Filipa Correia
Costa, João Manuel Rainha
Cardoso, Beatriz
Amorim, Cláudia
Silvério, Sara Isabel Cruz
Fernández-Lobato, María
Rodrigues, Joana Lúcia Lima Correia
Rodrigues, L. R.
Fructooligosaccharides (FOS)
Levansucrase (sacB)
One-step fermentation
Schwanniomyces occidentalis (Ffase-Leu196)
Zymomonas mobilis
Issue date24-Jun-2022
PublisherSpringer Nature
JournalApplied Microbiology and Biotechnology
CitationBraga, A., Gomes, D., Rainha, J. et al. Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4. Appl Microbiol Biotechnol 106, 4617–4626 (2022).
Abstract(s)Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated -fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4±1.6 g L1 of FOS, with a productivity of 1.53±0.03 g L1 h1 and a yield of 0.31±0.03 gFOS gsucrose1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process.
DescriptionThe online version contains supplementary material available at
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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