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TitleH. pylori phages: from genome release to hope for use as therapy
Author(s)Ferreira, Rute Vanessa Novais
Sousa, Cláudia Sofia Cunha
Presa, Eva
Pires, Diana Priscila Penso
Gonçalves, Raquel F. S.
Pinheiro, A. C.
Oleastro, Mónica
Azeredo, Joana
Figueiredo, Céu
Melo, Luís Daniel Rodrigues
Issue date8-Sep-2022
CitationFerreira, R.; Sousa, Cláudia; Presa, Eva; Pires, Diana P.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel Rodrigues, H. pylori phages: from genome release to hope for use as therapy. EHMSG 2022 35 th International Workshop on Helicobacter & Microbiota in Inflammation & Cancer. Glasgow, UK, Sep 8-10, 2022.
Abstract(s)The increasing antibiotic-resistant Helicobacter pylori infections worldwide and the ineffectiveness of treatments led the World Health Organization to designate clarithromycin-resistant H. pylori as a high-priority bacterium for antibiotic research and development. (Bacterio)phages, viruses that infect bacteria, showing effectiveness in the treatment of pathogenic bacteria, could be a promising alternative strategy in the fight against H. pylori infections. Material and methods In this work, a collection of 74 Portuguese H. pylori-clinical strains was used to screen for the presence of phage genes, using a new PCR-based method. Selected strains were subsequently sequenced and prophage isolation was attempted using UV radiation. Three phages were isolated, one of which was further characterized genetically and biologically. Results PCR-based detection indicated the presence of target phage sequences in 14 strains, and the induction strategies resulted in the release of a new phage. It presents a genome length of 31,162 bp with a G+C content of 37.1 %. This podovirus showed capability to form phage plaques in five strains, was stable under an in vitro gastric digestion model, and was able to maintain a H. pylori population at low levels for up to 24h post-infection. Conclusion The new PCR screening method proved to be very effective in the selection of strains carrying prophages, resulting in the isolation of a new H. pylori phage. This phage presented very promising characteristics in terms of stability and efficacy, being therefore a small step towards the future use of phage therapy in the fight against H. pylori infections.
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AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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