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https://hdl.handle.net/1822/79505
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreira, Rute Vanessa Novais | por |
dc.contributor.author | Sousa, Cláudia Sofia Cunha | por |
dc.contributor.author | Presa, Eva | por |
dc.contributor.author | Pires, Diana Priscila Penso | por |
dc.contributor.author | Gonçalves, Raquel F. S. | por |
dc.contributor.author | Pinheiro, A. C. | por |
dc.contributor.author | Oleastro, Mónica | por |
dc.contributor.author | Azeredo, Joana | por |
dc.contributor.author | Figueiredo, Céu | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.date.accessioned | 2022-09-14T08:43:31Z | - |
dc.date.available | 2022-09-14T08:43:31Z | - |
dc.date.issued | 2022-09-08 | - |
dc.identifier.citation | Ferreira, R.; Sousa, Cláudia; Presa, Eva; Pires, Diana P.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel Rodrigues, H. pylori phages: from genome release to hope for use as therapy. EHMSG 2022 35 th International Workshop on Helicobacter & Microbiota in Inflammation & Cancer. Glasgow, UK, Sep 8-10, 2022. | por |
dc.identifier.uri | https://hdl.handle.net/1822/79505 | - |
dc.description.abstract | The increasing antibiotic-resistant Helicobacter pylori infections worldwide and the ineffectiveness of treatments led the World Health Organization to designate clarithromycin-resistant H. pylori as a high-priority bacterium for antibiotic research and development. (Bacterio)phages, viruses that infect bacteria, showing effectiveness in the treatment of pathogenic bacteria, could be a promising alternative strategy in the fight against H. pylori infections. Material and methods In this work, a collection of 74 Portuguese H. pylori-clinical strains was used to screen for the presence of phage genes, using a new PCR-based method. Selected strains were subsequently sequenced and prophage isolation was attempted using UV radiation. Three phages were isolated, one of which was further characterized genetically and biologically. Results PCR-based detection indicated the presence of target phage sequences in 14 strains, and the induction strategies resulted in the release of a new phage. It presents a genome length of 31,162 bp with a G+C content of 37.1 %. This podovirus showed capability to form phage plaques in five strains, was stable under an in vitro gastric digestion model, and was able to maintain a H. pylori population at low levels for up to 24h post-infection. Conclusion The new PCR screening method proved to be very effective in the selection of strains carrying prophages, resulting in the isolation of a new H. pylori phage. This phage presented very promising characteristics in terms of stability and efficacy, being therefore a small step towards the future use of phage therapy in the fight against H. pylori infections. | por |
dc.language.iso | eng | por |
dc.rights | openAccess | por |
dc.title | H. pylori phages: from genome release to hope for use as therapy | por |
dc.type | conferenceAbstract | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.workshop.ehmsg.org/ | por |
dc.comments | CEB55729 | por |
oaire.citationConferencePlace | Glasgow, UK | por |
dc.date.updated | 2022-09-13T10:36:24Z | - |
dc.identifier.doi | 10.26355/mhd_20229_735 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.conferencePublication | EHMSG 2022 35 th International Workshop on Helicobacter & Microbiota in Inflammation & Cancer | por |
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document_55729_1.pdf | 107,22 kB | Adobe PDF | Ver/Abrir |