Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells

doi:10.3390/molecules27134001

Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/80295

Registo completo

Campo DC	Valor	Idioma
dc.contributor.author	Freitas, Ana Sofia	por
dc.contributor.author	Costa, Marta Sílvia Freitas	por
dc.contributor.author	Pontes, Olívia	por
dc.contributor.author	Seidel, Veronique	por
dc.contributor.author	Proença, M. Fernanda R. P.	por
dc.contributor.author	Cardoso, Susana M.	por
dc.contributor.author	Oliveira, Rui Pedro Soares de	por
dc.contributor.author	Baltazar, Fátima	por
dc.contributor.author	Almeida Aguiar, Cristina	por
dc.date.accessioned	2022-10-24T08:45:09Z	-
dc.date.available	2022-10-24T08:45:09Z	-
dc.date.issued	2022-06-22	-
dc.identifier.citation	Freitas, A.S.; Costa, M.; Pontes, O.; Seidel, V.; Proença, F.; Cardoso, S.M.; Oliveira, R.; Baltazar, F.; Almeida-Aguiar, C. Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells. Molecules 2022, 27, 4001. https://doi.org/10.3390/molecules27134001	por
dc.identifier.uri	https://hdl.handle.net/1822/80295	-
dc.description.abstract	Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.	por
dc.description.sponsorship	Ana Freitas acknowledge the financial support provided by national funds through FCT-Portuguese Foundation for Science and Technology (PD/BD/128276/2017), under the Doctoral Programme “Agricultural Production Chains–from fork to farm” (PD/00122/2012) and from the European Social Funds and the Regional Operational Programme Norte 2020. This study was also supported by CITAB research unit (UIDB/04033/2020) and by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P. An acknowledge also to the University of Aveiro and to Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) for financial support to the associated laboratory LAQV-REQUIMTE (project reference UIDB/50006/2020), through national funds and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER), within the PT2020 Partnership Agreement. We also acknowledge the financial support from University of Minho, Fundação para a Ciência e a Tecnologia (FCT) and FEDERCOMPETE for financial support through Centro de Química (UID/QUI/00686/2013 and UID/QUI/0686/2016), for the PhD grant awarded to Olívia Pontes (SFRH/BD/128850/2017). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network (RNRMN) and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT. This work has been funded by National funds, through the Foundation for Science and Technology (FCT)-project UIDB/50026/2020 and UIDP/50026/2020.	por
dc.language.iso	eng	por
dc.publisher	Multidisciplinary Digital Publishing Institute (MDPI)	por
dc.relation	info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F128276%2F2017/PT	por
dc.relation	PD/00122/2012	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04050%2F2020/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00686%2F2013/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F0686%2F2016/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F128850%2F2017/PT	por
dc.relation	REDE/1517/RMN/2005	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50026%2F2020/PT	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50026%2F2020/PT	por
dc.rights	openAccess	por
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	por
dc.subject	Propolis	por
dc.subject	Fractionation	por
dc.subject	Phenolic compounds	por
dc.subject	Pectolinarigenin	por
dc.subject	Cytotoxic activity	por
dc.subject	Renal cell carcinoma	por
dc.title	Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells	por
dc.type	article	por
dc.peerreviewed	yes	por
dc.relation.publisherversion	https://www.mdpi.com/1420-3049/27/13/4001	por
oaire.citationStartPage	1	por
oaire.citationEndPage	13	por
oaire.citationIssue	13	por
oaire.citationVolume	27	por
dc.date.updated	2022-07-08T11:55:27Z	-
dc.identifier.eissn	1420-3049	-
dc.identifier.doi	10.3390/molecules27134001	por
dc.identifier.pmid	35807247	por
dc.subject.wos	Science & Technology	por
sdum.journal	Molecules	por
oaire.version	VoR	por
dc.identifier.articlenumber	4001	por
Aparece nas coleções:	ICVS - Artigos em revistas internacionais / Papers in international journals CBMA - Artigos/Papers