Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81605
Título: | Fibroblasts Impair Migration and Antitumor Activity of NK-92 Lymphocytes in a Melanoma-on-Chip Model |
Autor(es): | Iaia, Ilenia Brancato, Virginia Caballero, David Reis, R. L. Aglietta, Massimo Sangiolo, Dario Kundu, Subhas C |
Palavras-chave: | Cancer Microfabrication Microfluidics Organ-on-a-chip Tumor-on-chip Adoptive cell therapy Melanoma |
Data: | 2023 |
Editora: | MDPI |
Revista: | Bioengineering |
Citação: | Iaia, I.; Brancato, V.; Caballero, D.; Reis, R.L.; Aglietta, M.; Sangiolo, D.; Kundu, S.C. Fibroblasts Impair Migration and Antitumor Activity of NK-92 Lymphocytes in a Melanoma-on-Chip Model. Bioengineering 2023, 10, 52. https://doi.org/10.3390/bioengineering10010052 |
Resumo(s): | Adoptive cell therapy in solid tumors, such as melanoma, is impaired, but little is known about the role that the fibroblasts present in the tumor microenvironment could exert. However, the mechanism at play is not well understood, partly due to the lack of relevant pre-clinical models. Three-dimensional culture and microfluidic chips are used to recapitulate the dynamic interactions among different types of cells in the tumor microenvironment in controlled and physiological settings. In this brief report, we propose a reductionist melanoma-on-a-chip model for evaluating the essential role of fibroblasts in the antitumor activity of lymphocytes. To this end, 3D melanoma spheroids were monocultured and co-cultured with human dermal fibroblasts and the NK-92 cell migration towards the tumor compartment was tested in a commercially available microfluidic device. Utilizing confocal microscopy, we observed the different recruitment of NK-92 cells in the presence and absence of fibroblasts. Our results show that fibroblastsâ presence inhibits immune effector recruiting by exploiting a 3D pre-clinical tumor model. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/81605 |
DOI: | 10.3390/bioengineering10010052 |
ISSN: | 2306-5354 |
Versão da editora: | https://www.mdpi.com/2306-5354/10/1/52 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20873-bioengineering-melanoma-chip.pdf | 748 kB | Adobe PDF | Ver/Abrir |