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https://hdl.handle.net/1822/82735
Título: | Echinacea purpurea fractions represent promising plant-based anti-inflammatory formulations |
Autor(es): | Vieira, Sara Filipa Fontoura Gonçalves, Samuel M. Gonçalves, Virgínia M. F. Llaguno, Carmen P. Macías, Felipe Tiritan, Maria Elizabeth Cunha, Cristina Carvalho, Agostinho Reis, R. L. Ferreira, Helena Neves, N. M. |
Palavras-chave: | Alkylamides Echinacea purpurea extracts Fractions Human primary macrophages Inflammation Phenols/carboxylic acids phenols carboxylic acids |
Data: | Fev-2023 |
Editora: | MDPI |
Revista: | Antioxidants |
Citação: | Vieira S. F., Gonçalves S. M., Gonçalves V. M. F., Llaguno C. P., Macías F., Tiritan M. E., Cunha C., Carvalho A., Reis R. L., Ferreira H., Neves N. M. Echinacea purpurea Fractions Represent Promising Plant-Based Anti-Inflammatory Formulations, Antioxidants, Vol. 12, Issue 2, pp. 1-25, doi:10.3390/antiox12020425, 2023 |
Resumo(s): | Echinacea purpurea is traditionally used in the treatment of inflammatory diseases. Therefore, we investigated the anti-inflammatory capacity of E. purpurea dichloromethanolic (DE) and ethanolic extracts obtained from flowers and roots (R). To identify the class of compounds responsible for the strongest bioactivity, the extracts were fractionated into phenol/carboxylic acid (F1) and alkylamide fraction (F2). The chemical fingerprint of bioactive compounds in the fractions was evaluated by LC-HRMS. E. purpurea extracts and fractions significantly reduced pro-inflammatory cytokines (interleukin 6 and/or tumor necrosis factor) and reactive oxygen and nitrogen species (ROS/RNS) production by lipopolysaccharide-stimulated primary human monocyte-derived macrophages. Dichloromethanolic extract obtained from roots (DE-R) demonstrated the strongest anti-inflammatory activity. Moreover, fractions exhibited greater anti-inflammatory activity than whole extract. Indeed, alkylamides must be the main compounds responsible for the anti-inflammatory activity of extracts; thus, the fractions presenting high content of these compounds presented greater bioactivity. It was demonstrated that alkylamides exert their anti-inflammatory activity through the downregulation of the phosphorylation of p38, ERK 1/2, STAT 3, and/or NF-κB signaling pathways, and/or downregulation of cyclooxygenase 2 expression. E. purpurea extracts and fractions, mainly DE-R-F2, are promising and powerful plant-based anti-inflammatory formulations that can be further used as a basis for the treatment of inflammatory diseases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/82735 |
DOI: | 10.3390/antiox12020425 |
ISSN: | 2076-3921 |
Versão da editora: | https://www.mdpi.com/2076-3921/12/2/425 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20896-antioxidants-12-00425.pdf | 3,47 MB | Adobe PDF | Ver/Abrir |