Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/83602
Título: | Cerebral malaria model applying human brain organoids |
Autor(es): | Pedrosa, Ana Rita Silva Campos, Jonas Oliveira Fernandes, Aline Marie Silva, Miguel Calçada, Carla Sofia Martins Marote, Ana Maria Franco Aveiro Martinho, Olga Veiga, Maria Isabel Rodrigues, L. R. Salgado, A. J. Ferreira, Pedro Eduardo Mendes |
Palavras-chave: | Cerebral malaria Brain organoids Transcriptome Human iPSCs Secretome HBMEC activation |
Data: | 16-Mar-2023 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Cells |
Citação: | Pedrosa, Ana; Campos, Jonas; Fernandes, Aline Marie; Silva, Miguel; Calçada, Carla; Marote, Ana; Martinho, Olga; Veiga, Maria Isabel; Rodrigues, Lígia R.; Salgado, António José; Ferreira, Pedro Eduardo, Cerebral malaria model applying human brain organoids. Cells, 12(7), 984, 2023 |
Resumo(s): | Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood–brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/83602 |
DOI: | 10.3390/cells12070984 |
e-ISSN: | 2073-4409 |
Versão da editora: | https://www.mdpi.com/journal/cells |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56129_1.pdf | 5,76 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons