Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/9454
Título: | Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula |
Autor(es): | Almeida, Carina Azevedo, N. F. Iversen, C. Fanning, S. Keevil, C. W. Vieira, M. J. |
Data: | Mai-2009 |
Editora: | American Society for Microbiology (ASM) |
Revista: | Applied and Environmental Microbiology |
Citação: | "Applied and Environmental Microbiology". ISSN 0099-2240. 75:9 (May 2009) 2925-2930. |
Resumo(s): | Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/9454 |
DOI: | 10.1128/AEM.02470-08 |
ISSN: | 0099-2240 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Almeida_AEM.pdf | 959,71 kB | Adobe PDF | Ver/Abrir |