Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1934
Título: | Partial purification of penicillin acylase from Escherichia Coli in poly(ethylene glycol)-sodium citrate aqueous two-phase systems |
Autor(es): | Marcos, João Carlos Fonseca, Luís Pina Ramalho, Maria Teresa Cabral, J. M. S. |
Palavras-chave: | Aqueous two-phase systems Poly(ethyleneglycol) - sodium citrate systems Partitioning Purification Penicillin acylase poly(ethylene glycol)-sodium citrate |
Data: | 1999 |
Editora: | Elsevier 1 |
Revista: | Journal of Chromatography B |
Citação: | "Journal of Chromatography B: Biomedical Sciences and Applications". ISSN 1570-0232. 734:1 (1999) 15-22. |
Resumo(s): | Studies on the partition and purification of penicillin acylase from osmotic shock extract Escherichia coli were performed in poly (ethylene glycol)- citrate systems. Both partition behavior of the enzyme and total protein are similar to those described in other reports increasing ,with pH and tie-line length and decreasing with PEG molecular weight . However, some selectivity could be attained with PEG 1000 systems and long tie-line at pH 6.9. In these conditions 2.6 fold purification with 83% yield were achieved. Influence of pH on partition shows that is the composition of the system and not the net charge of the enzyme that determine the behaviour in these conditions. Addition of NaCl to PEG 3350 systems significantly increases the partition of the enzyme. Althought protein partition also increased, purification conditions were possible with 1.5 M NaCl where 5.7 fold purification and 85% yield was obtained. This was possible due to the higher hydrophobicity of the enzyme compared to that of most of contaminants proteins. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1934 |
DOI: | 10.1016/S0378-4347(99)00319-9 |
ISSN: | 0378-4347 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ParPurPenAcText_RepUM.pdf | Documento principal | 124,72 kB | Adobe PDF | Ver/Abrir |
ParPurPenAcFig_RepUM.pdf | Figuras | 95,95 kB | Adobe PDF | Ver/Abrir |