Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/19933
Título: | Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography |
Autor(es): | Santos, Maria João Teixeira, J. A. Rodrigues, L. R. |
Palavras-chave: | Whey proteins Anion-exchange chromatography Separation Isolation |
Data: | 2012 |
Editora: | Elsevier 1 |
Revista: | Separation and Purification Technology |
Resumo(s): | A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl). The proposed method was found to be suitable to fractionate the major whey proteins from the WPC80 in different fractions, namely one fraction containing all the a-Lactalbumin and immunoglobulins; another fraction containing all the bovine serum albumin; and two distinct fractions each containing a different variant of b-Lactoglobulin. A 60.5% (w/w) recovery of the two main b-Lactoglobulin variants was obtained. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/19933 |
DOI: | 10.1016/j.seppur.2012.02.030 |
ISSN: | 1383-5866 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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santos_2012.pdf | 540,34 kB | Adobe PDF | Ver/Abrir |