Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64150
Título: | (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line |
Autor(es): | Souto, Eliana B. Zielinska, Aleksandra Souto, Selma B. Durazzo, Alessandra Lucarini, Massimo Santini, Antonello Silva, Amélia M. Atanasov, Atanas G. Marques, Conrado Andrade, Luciana N. Severino, Patricia |
Palavras-chave: | (+)-limonene 1 2-epoxide monoterpene Imwitor 900K-SLN lipid peroxidation cytotoxicity HaCaT cell line (+)-limonene 1,2-epoxide |
Data: | 20-Fev-2020 |
Editora: | MDPI |
Revista: | International Journal of Molecular Sciences |
Citação: | Souto, Eliana; Zielinska, Aleksandra; Souto, Selma B.; Durazzo, Alessandra; Lucarini, Massimo; Santini, Antonello; Silva, Amélia M.; Atanasov, Atanas G.; Marques, Conrado; Andrade, Luciana N.; Severino, Patricia, (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line. International Journal of Molecular Sciences, 21(4), 1449, 2020 |
Resumo(s): | In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64150 |
ISBN: | 1424-6783 |
DOI: | 10.3390/ijms21041449 |
ISSN: | 1422-0067 |
e-ISSN: | 1661-6596 |
Versão da editora: | http://www.mdpi.com/journal/ijms |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53510_1.pdf | 1,19 MB | Adobe PDF | Ver/Abrir |