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https://hdl.handle.net/1822/65928
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Campo DC | Valor | Idioma |
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dc.contributor.author | Trobajo-Sanmartín, Camino | por |
dc.contributor.author | Ezpeleta, Guillermo | por |
dc.contributor.author | Pais, Célia | por |
dc.contributor.author | Eraso, Elena | por |
dc.contributor.author | Quindós, Guillermo | por |
dc.date.accessioned | 2020-07-10T08:48:33Z | - |
dc.date.available | 2020-07-10T08:48:33Z | - |
dc.date.issued | 2018-09-29 | - |
dc.identifier.citation | Trobajo-Sanmartín, C., Ezpeleta, G., Pais, C. et al. Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates. BMC Genomics 19, 718 (2018). https://doi.org/10.1186/s12864-018-5065-3 | por |
dc.identifier.issn | 1471-2164 | por |
dc.identifier.uri | https://hdl.handle.net/1822/65928 | - |
dc.description.abstract | Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated. | por |
dc.description.sponsorship | This work was supported by the Consejeria de Educacion, Universidades e Investigacion of the Gobierno Vasco-Eusko Jaurlaritza [GIC 15/78 IT-990-16] and the Universidad del Pais Vasco/Euskal Herriko Unibertsitatea [UFI 11/25]. CTS has been supported by a research grant of the Fundacion Jesus de Gangoiti Barrera. | por |
dc.language.iso | eng | por |
dc.publisher | BioMed Central (BMC) | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Candida parapsilosis | por |
dc.subject | Candidiasis, Invasive | por |
dc.subject | DNA, Fungal | por |
dc.subject | Genotyping Techniques | por |
dc.subject | Humans | por |
dc.subject | Multiplex Polymerase Chain Reaction | por |
dc.subject | Mycological Typing Techniques | por |
dc.subject | Sensitivity and Specificity | por |
dc.subject | Microsatellite Repeats | por |
dc.subject | Candida parapsilosis sensu stricto | por |
dc.subject | Genotyping technique | por |
dc.subject | Microsatellites repeats | por |
dc.subject | Multiplex PCR | por |
dc.subject | Specificity | por |
dc.subject | Reproducibility of results | por |
dc.title | Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-018-5065-3 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 19 | por |
dc.identifier.doi | 10.1186/s12864-018-5065-3 | por |
dc.identifier.pmid | 30268088 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | BMC Genomics | por |
oaire.version | VoR | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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s12864-018-5065-3.pdf | 1,26 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons