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https://hdl.handle.net/1822/77002
Título: | Prevalence of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from Northern Portugal |
Autor(es): | Oliveira, Ricardo Pinho, Eva Almeida, Gonçalo Azevedo, Nuno Filipe Almeida, Carina Manuela Fernandes |
Data: | 18-Nov-2021 |
Citação: | Oliveira, Ricardo; Pinho, Eva; Almeida, Gonçalo; Azevedo, Nuno F.; Almeida, Carina, Prevalence of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from Northern Portugal. Dare2Change - Innovation-Driven Agrifood Business. Porto, Portugal, Nov 18-19, 68, 2021. |
Resumo(s): | Staphylococcus aureus and its enterotoxins (SEs) are a serious and costly concern for milk industry and public health [1]. This work aimed to characterize the prevalence of S. aureus and enterotoxins in raw milk collected in the main dairy basin region of Portugal mainland. The presence of S. aureus was confirmed in 53% of refrigerated raw milk samples collected from the bulk tank of 100 dairy farms according to the ISO standards. Nonetheless, S. aureus was always below 106 CFU/mL, the minimal concentration expected for enterotoxin production [2]. The presence of enterotoxins- (sea, seb, sec, sed, see, seg, seh, sei, sej, sep, ser) and methicillin resistance-encoding genes (mecA and mecC) was evaluated by PCR. Five isolates were found to be methicillin-resistant Staphylococcus aureus (MRSA) and 29 isolates contained enterotoxin-encoding genes. One isolate was positive for sea, 3 isolates were positive for seh, 4 isolates were positive for sec, 25 isolates were positive for sei and 26 isolates were positive for seg. Consistent with other reports, seg and sei coexisted in most isolates, seg was only detected independently of the other gene in one isolate [3, 4]. Sec was only found in conjunction with seg and sei. Seh was detected alone or together with sea. Overall, the occurrence of non-typical enterotoxin genes (seg, seh and sei) was higher than the top five genes (sea-see). The detection of SEs (SEA-SEE), according to the EU-RL standard, revealed one positive sample. Interestingly, S. aureus was not detected on the positive sample, demonstrating that SEs can be present without requiring the presence of the bacteria [5]. These results suggest that raw milk can be an important source of MRSA, enterotoxigenic S. aureus and enterotoxins. Surveillance and postharvest handling practices might be crucial to prevent the spread along the food chain. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/77002 |
Versão da editora: | https://dare2change.pt/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55478_1.pdf | 330,71 kB | Adobe PDF | Ver/Abrir |