Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/83902
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Azinheiro, Sarah | por |
dc.contributor.author | Rodríguez-López, Pedro | por |
dc.contributor.author | Lozano-León, Antonio | por |
dc.contributor.author | Guedes, Hugo | por |
dc.contributor.author | Regal, Patricia | por |
dc.contributor.author | Franco, Carlos M. | por |
dc.contributor.author | Cepeda, Alberto | por |
dc.contributor.author | Teixeira, Maria Pilar Araújo | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.contributor.author | Silva, Daniela | por |
dc.contributor.author | Fernández, Ana | por |
dc.contributor.author | Faria, Márcia | - |
dc.contributor.author | Roumani, Foteini | - |
dc.contributor.author | Juan Herrera, | - |
dc.contributor.author | Prado, Marta | - |
dc.contributor.author | López-Cabo, Marta | - |
dc.contributor.author | Garrido-Maestu, Alejandro | - |
dc.date.accessioned | 2023-04-12T09:57:35Z | - |
dc.date.available | 2023-04-12T09:57:35Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.citation | Azinheiro, Sarah; Rodríguez-López, Pedro; Lozano-León, Antonio; Guedes, Hugo; Regal, Patricia; Franco, Carlos M.; Cepeda, Alberto; Teixeira, Pilar; Melo, Luís Daniel Rodrigues; Silva, Daniela; Fernández, Ana; Faria, Márcia; Roumani, Foteini; Herrera, Juan; Prado, Marta; López-Cabo, Marta; Garrido-Maestu, Alejandro, Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product. Food Control, 150(109769), 2023 | por |
dc.identifier.issn | 0956-7135 | por |
dc.identifier.uri | https://hdl.handle.net/1822/83902 | - |
dc.description.abstract | Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as almost complete agreement. | por |
dc.description.sponsorship | This work was financially supported by the Seafood Age project, which was co-financed by the Interreg Atlantic Area Program (EAPA_758/2018) though the European Development Fund (ERDF). Mrs. Sarah Azinheiro was financed by a Ph.D. grant from the Fundação para a Ciência e a Tecnologia (SFRH/BD/140396/2018). Dr. Alejandro Garrido-Maestu and Luís D. R. Melo acknowledge funding from the Fundação para a Ciência e Tecnologia through the Scientific Employment Stimulus Program (2021.02810.CEECIND and 2021.00221.CEECIND, respectively). This study was supported by the Fundação para a Ciência e a Tecnologia (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. | - |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F140396%2F2018/PT | - |
dc.relation | 2021.02810.CEECIND | - |
dc.relation | 2021.00221.CEECIND | - |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | - |
dc.rights | openAccess | por |
dc.subject | Interlaboratory validation | por |
dc.subject | qPCR | por |
dc.subject | Ready-to-eat | por |
dc.subject | Fish products | por |
dc.subject | Alternative methods | por |
dc.subject | Listeria monocytogenes | por |
dc.title | Interlaboratory validation of a multiplex qPCR method for the detection of listeria monocytogenes in a ready-to-eat seafood product | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/food-control | por |
dc.comments | CEB56150 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 150 | por |
dc.date.updated | 2023-04-11T09:42:20Z | - |
dc.identifier.doi | 10.1016/j.foodcont.2023.109769 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Food Control | por |
oaire.version | P | - |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56150_1.pdf | 2,44 MB | Adobe PDF | Ver/Abrir |