Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/13141
Título: | Influence of mechanical agitation on cutinases and protease activity towards polyamide substrates |
Autor(es): | Silva, Carla Manuela Pereira Marinho da Araújo, Rita Casal, Margarida Gübitz, Georg M. Paulo, Artur Cavaco |
Palavras-chave: | Polyamide Cutinase Protease Hydrolysis |
Data: | Jun-2007 |
Editora: | Elsevier 1 |
Revista: | Enzyme and Microbial Technology |
Resumo(s): | Two polyamide 6,6 substrates with different constructions, namely a model substrate and a fabric, were hydrolyzed using native cutinase and L182A cutinase mutant (from Fusarium solani pisi) and a protease (subtilisin from Bacillus sp.). The catalytic efficiency of these enzymes, measured in terms of hydrolysis products release, was measured for both substrates and the protease released five times more amines to the bath treatment. The L182A cutinase mutant showed higher activity when compared with the native enzyme. All enzymes have shown activity additive effects with higher levels of mechanical agitation for polyamide fabrics. The results achieved are of paramount importance on the design of a process of enzymatic functionalization of polyamide. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/13141 |
DOI: | 10.1016/j.enzmictec.2006.09.001 |
ISSN: | 0141-0229 |
Versão da editora: | http://www.sciencedirect.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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INFLUENCE OF MECHANICAL AGITATION ON CUTINASES AND PROTEASE ACTIVITY TOWARDS POLYAMIDE SUBSTRATES.pdf | Influence of mechanical agitation on cutinases and protease activity towards polyamide substrates | 898,83 kB | Adobe PDF | Ver/Abrir |