Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1399
Título: | Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4 |
Autor(es): | Venâncio, Armando Domingues, Lucília Lima, Nelson |
Data: | 1999 |
Editora: | Wiley |
Revista: | Journal of Basic Microbiology |
Citação: | "Journal of basic microbiology". ISSN 0233-111X. 39:1 (1999) 37-41. |
Relatório da Série N.º: | 1999;23 |
Resumo(s): | A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10³ transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1399 |
ISSN: | 0233-111X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1999-23[1].pdf | 233,97 kB | Adobe PDF | Ver/Abrir |