Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14273
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Baran, E. T. | - |
dc.contributor.author | Ozer, Nazmi | - |
dc.contributor.author | Hasirci, Vasif | - |
dc.date.accessioned | 2011-11-11T16:14:18Z | - |
dc.date.available | 2011-11-11T16:14:18Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1570-0232 | por |
dc.identifier.uri | https://hdl.handle.net/1822/14273 | - |
dc.description.abstract | In the present study antileukemic enzyme L-asparaginase (ASNase) and catalase (as a model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG ) by using ligand-immobilized affinity column systems L-asparagine- 2 Sepharose CL-4B and Procion red-Sepharose CL-4B, respectively. Studies on change of specific activity with modification time showed negligible differences between batches of modified catalase. Modification of ASNase for 1 h resulted in 50.2% recovery of the specific activity and the attachment of 69 molecules of PEG per molecule of ASNase forming ‘PEGylated 2 ASNase’. Sequential modification of ASNase by activated PEG and heparin resulted in coupling of about nine molecules of heparin per molecule of PEGylated ASNase. Intravenous (i.v.) administration of PEG -modified ASNase showed prolonged 2 presence in the blood circulation and no adverse effects or symptoms of anaphylaxis were observed in presensitized mice. | por |
dc.description.sponsorship | (METU AFP-99-06-02-23) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Enzymes | por |
dc.subject | L-asparaginase | por |
dc.title | Solid-phase enzyme modification via affinity chromatography | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 311 | por |
oaire.citationEndPage | 322 | por |
oaire.citationIssue | 2 | por |
oaire.citationTitle | Journal of chromatography B : analytical technologies in the biomedical and life sciences | por |
oaire.citationVolume | 794 | por |
dc.identifier.doi | 10.1016/S1570-0232(03)00487-2 | por |
dc.identifier.pmid | 12954382 | por |
sdum.journal | Journal of Chromatography B : Analytical Technologies in the Biomedical and Life Sciences | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |