Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/18355
Título: | Spatio-temporal population structuring and genetic diversity retention in depleted Atlantic Bluefin tuna of the Mediterranean Sea |
Autor(es): | Riccioni, Giulia Landi, Monica Ferrara, Giorgia Milano, Ilaria Cariani, Alessia Zane, Lorenzo Sella, Massimo Barbujani, Guido Tinti, Fausto |
Palavras-chave: | Ancient DNA Effective population size Genetic structure Large pelagic Thunnus thynnus Large pelagic fishes |
Data: | 2-Fev-2010 |
Editora: | National Academy of Sciences |
Revista: | Proceedings of the National Academy of Sciences |
Resumo(s): | There are a number of challenges associated with designing nanoparticles for medical applications. We define two challenges here: (i) conventional targeting against up-regulated cell surface antigens is limited by heterogeneity in expression, and (ii) previous studies suggest that the optimal size of nanoparticles designed for systemic delivery is approximately 50–150 nm, yet this size range confers a high surface area-to-volume ratio, which results in fast diffusive drug release. Here, we achieve spatial control by biopanning a phage library to discover materials that target abundant vascular antigens exposed in disease. Next, we achieve temporal control by designing 60-nm hybrid nanoparticles with a lipid shell interface surrounding a polymer core, which is loaded with slow-eluting conjugates of paclitaxel for controlled ester hydrolysis and drug release over approximately 12 days. The nanoparticles inhibited human aortic smooth muscle cell proliferation in vitro and showed greater in vivo vascular retention during percutaneous angioplasty over nontargeted controls. This nanoparticle technology may potentially be used toward the treatment of injured vasculature, a clinical problem of primary importance. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/18355 |
DOI: | 10.1073/pnas.0908281107 |
ISSN: | 1091-6490 |
Versão da editora: | http://www.pnas.org/content/107/5/2102.full.pdf |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Thunnus thynnus_PNAS.pdf Acesso restrito! | Manuscript of the paper | 199,95 kB | Adobe PDF | Ver/Abrir |