Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22165
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Campo DC | Valor | Idioma |
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dc.contributor.author | Seyedsayamdost, Mohammad R. | - |
dc.contributor.author | Cleto, S. | - |
dc.contributor.author | Carr, Gavin | - |
dc.contributor.author | Vlamakis, Hera | - |
dc.contributor.author | Vieira, M. J. | - |
dc.contributor.author | Kolter, Roberto | - |
dc.contributor.author | Clardy, Jon | - |
dc.date.accessioned | 2013-01-03T14:30:15Z | - |
dc.date.available | 2013-01-03T14:30:15Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0002-7863 | por |
dc.identifier.issn | 1520-5126 | por |
dc.identifier.uri | https://hdl.handle.net/1822/22165 | - |
dc.description.abstract | Studying the evolutionary history underlying the remarkable structures and biological activities of natural products has been complicated by not knowing the functions they have evolved to fulfill. Siderophores - soluble, low molecular weight compounds - have an easily understood and measured function: acquiring iron from the environment. Bacteria engage in a fierce competition for acquiring iron, which rewards the production of siderophores that bind iron tightly and cannot be used or pirated by competitors. The structures and biosyntheses of 'odd' siderophores can reveal the evolutionary strategy that led to their creation. Here, we here report a new Serratia strain that produces serratiochelin and an analog of serratiochelin. A genetic approach located the serratiochelin gene cluster, and targeted mutations in several genes implicated in serratiochelin biosynthesis were generated. Bioinformatic analyses and mutagenesis results demonstrate that genes from two well known siderophore clusters, the Escherichia coli enterobactin cluster and the Vibrio cholerae vibriobactin cluster, were shuffled to produce a new siderophore biosynthetic pathway. These results highlight how modular siderophore gene clusters can be mixed and matched during evolution to generate structural diversity in siderophores. | por |
dc.description.sponsorship | This work was supported by the National Institutes of Health (Grants GM82137 to R.K., and AI057159 and GM086258 to J.C.). M.R.S. acknowledges support from the NIH Pathway to Independence Award (Grant 1K99 GM098299-01). S.C. and M.J.V. acknowledge support from the Portuguese Foundation for Science and Technology (PhD Grant SFRH/BD/38298/2007 to S.C.; Project PTDC/EBB-EBI/104263/2008 to M.J.V.). | por |
dc.language.iso | eng | por |
dc.publisher | American Chemical Society | por |
dc.rights | openAccess | por |
dc.title | Mixing and matching siderophore clusters: structure and biosynthesis of serratiochelins from Serratia sp. v4 | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 13550 | por |
oaire.citationEndPage | 13553 | por |
oaire.citationIssue | 33 | por |
oaire.citationTitle | Journal of the American Chemical Society | por |
oaire.citationVolume | 134 | por |
dc.identifier.doi | 10.1021/ja304941d | por |
dc.identifier.pmid | 22830960 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of the American Chemical Society | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ja304941d.pdf | 677,61 kB | Adobe PDF | Ver/Abrir |