Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/22773

TitleGene knockdown in Paracoccidioides brasiliensis using antisense RNA
Author(s)Menino, João F.
Almeida, A. J.
Rodrigues, Fernando José dos Santos
KeywordsParacoccidioides brasiliensis
Molecular techniques
aRNA technology
Gene knockdown
ATMT
Issue date2012
PublisherSpringer
JournalMethods in Molecular Biology
Abstract(s)Paracoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) technology taking advantage of an Agrobacterium tumefaciens-mediated transformation (ATMT) system. Together, these techniques represent a reliable toolbox that can be employed for functional genetic analysis of putative virulence factors and morphogenic regulators, aiming to the identification of new potential drug targets.
TypeBook part
URIhttps://hdl.handle.net/1822/22773
ISBN978-1-61779-538-1
DOI10.1007/978-1-61779-539-8_12
ISSN1064-3745
Publisher versionhttp://link.springer.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Livros e capítulos de Livros / Books and book chapters

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