Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22773
Título: | Gene knockdown in Paracoccidioides brasiliensis using antisense RNA |
Autor(es): | Menino, João F. Almeida, A. J. Rodrigues, Fernando José dos Santos |
Palavras-chave: | Paracoccidioides brasiliensis Molecular techniques aRNA technology Gene knockdown ATMT |
Data: | 2012 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Resumo(s): | Paracoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) technology taking advantage of an Agrobacterium tumefaciens-mediated transformation (ATMT) system. Together, these techniques represent a reliable toolbox that can be employed for functional genetic analysis of putative virulence factors and morphogenic regulators, aiming to the identification of new potential drug targets. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/22773 |
ISBN: | 978-1-61779-538-1 |
DOI: | 10.1007/978-1-61779-539-8_12 |
ISSN: | 1064-3745 |
Versão da editora: | http://link.springer.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Menino JF_Meth Molec Biol Book 2012 pp.pdf | Uncorrected proof | 367,15 kB | Adobe PDF | Ver/Abrir |