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https://hdl.handle.net/1822/24081
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Campo DC | Valor | Idioma |
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dc.contributor.author | Yan, Leping | - |
dc.contributor.author | Correia, Cristina | - |
dc.contributor.author | Silva-Correia, Joana | - |
dc.contributor.author | Caridade, S. G. | - |
dc.contributor.author | Fernandes, E. M. | - |
dc.contributor.author | Mano, J. F. | - |
dc.contributor.author | Sousa, R. A. | - |
dc.contributor.author | Oliveira, Joaquim M. | - |
dc.contributor.author | Oliveira, A. L. | - |
dc.contributor.author | Reis, R. L. | - |
dc.date.accessioned | 2013-05-16T17:24:40Z | - |
dc.date.available | 2013-05-16T17:24:40Z | - |
dc.date.issued | 2012-09 | - |
dc.date.submitted | 2012-09 | - |
dc.identifier.citation | Yan L. - P. Yan L. P., Correia C., Silva-Correia J., Caridade S. G., Fernandes E. M., Mano J. F., Sousa R. A., Oliveira J. M., Oliveira A. L., Reis R. L. Preparation and characterization of macro/micro porous silk fibroin /nano-sized calcium phosphate scaffolds for bone tissue engineering, Journal of tissue engineering and regenerative medicine, Vol. 6, Issue Suppl.1, pp. 181, doi:10.1002/term.1586, 2012 | - |
dc.identifier.issn | 1932-6254 | por |
dc.identifier.uri | https://hdl.handle.net/1822/24081 | - |
dc.description.abstract | Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combination of salt-leaching/freeze-drying methods. Thermal gravimetric analysis results were able to demonstrated that the silk/nano-CaP scaffolds maintained 64–87% of incorporated CaP after salt-leaching. Dynamic mechanical analysis showed that storage modulus of the 16% formulation was significantly higher than all remaining groups. The porosity of silk/nano-CaP scaffolds assessed using Micro-Computed Tomography decreased from 79.8% to 63.6% with increasing CaP incorporated until 16%. By soaking the scaffolds in Simulated Body Fluid for 7 days, cauliflower-like apatite clusters were observed on the surface of both macro and micro pores of 16% and 25% formulations, which was not observed in 4 and 8% formulations. 16% silk/nano-CaP scaffolds were further chosen for in vitro cytotoxicity and biocompatibility assays. Both silk and 16% silk/nano-CaP scaffolds were non-cytotoxic and promoted cell adhesion and proliferation to a similar extent. These results indicated that the 16% silk/ nano-CaP scaffolds could be a good candidate for bone tissue engineering. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | openAccess | por |
dc.subject | Bone | por |
dc.subject | Silk fibroin | por |
dc.title | Development of macro/micro porous silk fibroin scaffolds with nano-sized calcium phosphate particles for bone tissue engineering | por |
dc.type | conferenceAbstract | - |
dc.peerreviewed | yes | por |
dc.comments | http://www.3bs.uminho.pt/node/15891 | por |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | . | por |
sdum.event.type | conference | por |
oaire.citationStartPage | 181 | por |
oaire.citationEndPage | 181 | por |
oaire.citationIssue | Suppl.1 | - |
oaire.citationTitle | Journal of tissue engineering and regenerative medicine | por |
oaire.citationVolume | 6 | - |
dc.date.updated | 2013-04-16T13:15:13Z | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Tissue Engineering and Regenerative Medicine | por |
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15891-1349027738_Abstract of TERM 2012.Vienna 181.pdf | 60,02 kB | Adobe PDF | Ver/Abrir |