Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/25923
Título: | Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
Autor(es): | Shimanovich, Ulyana Eliaz, Dror Zigdon, Sally Volkov, Vadim Aizer, Adva Paulo, Artur Cavaco Michaeli, S. Shav-Tal, Yaron Gedanken, Aharon |
Data: | 2013 |
Editora: | Royal Society of Chemistry |
Revista: | Journal of Materials Chemistry B |
Resumo(s): | In the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. Using PVA coating of the RNA–BSA spheres we have achieved a significant increase in the number of microspheres penetrating mammalian cells. The mechanism of RNA encapsulation and the structure of the RNA–BSA complex are reported. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25923 |
DOI: | 10.1039/C2TB00012A |
ISSN: | 2050-7518 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2013 Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method.pdf | 604,16 kB | Adobe PDF | Ver/Abrir |