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https://hdl.handle.net/1822/28183
Título: | Enhanced cell affinity of chitosan membranes mediated by superficial cross-linking : a straightforward method attainable by standard laboratory procedures |
Autor(es): | Rodríguez-Velázquez, Eustolia Silva, Maite Taboada, Pablo Mano, J. F. Suárez-Quintanilla, D. Alatorre-Meda, Manuel |
Palavras-chave: | Biomaterials Cell culture Chemical vapor deposition Chitosan films |
Data: | 2014 |
Editora: | American Chemical Society |
Revista: | Biomacromolecules |
Citação: | Rodríguez-Velázquez E.;, Silva M.;, Taboada P., Mano J. F., Suárez-Quintanilla D., Alatorre-Meda M. Enhanced Cell Affinity of Chitosan Membranes Mediated by Superficial Cross-Linking: A Straightforward Method Attainable by Standard Laboratory Procedures, Biomacromolecules, Vol. 15, pp. 291-301, doi:dx.doi.org/10.1021/bm401541v, 2013 |
Resumo(s): | It is well accepted that the surface modification of biomaterials can improve their biocompatibility. In this context, techniques like ion etching, plasma-mediated chemical functionalization, electrospinning, and contact microprinting have successfully been employed to promote the cell adhesion and proliferation of chitosan (CH) substrates. However, they prove to be time-consuming, highly dependent on environmental conditions, and/or limited to the use of expensive materials and sophisticated instruments not accessible to standard laboratories, hindering to a high extent their straightforward application. Filling this gap, this paper proposes the superficial cross-linking of CH as a much simpler and accessible means to modify its superficial properties in order to enhance its cellular affinity. CH membranes were prepared by solvent casting followed by a cross-linking step mediated by the chemical vapor deposition (CVD) of glutaraldehyde (GA). The membranes were characterized against non- and solution cross-linked membranes in terms of their mechanical/surface properties and biological performance. Among others, the CVD membranes proved (i) to be more mechanically stable against cell culture and sterilization than membranes cross-linked in solution and (ii) to prompt the adherence and sustained proliferation of healthy cells to levels even superior to commercial tissue culture plates (TCPs). Accordingly, the CVD cross-linking approach was demonstrated to be a simple and cost-effective alternative to the aforementioned conventional methods. Interestingly, this concept can also be applied to other biomaterials as long as GA (or other volatile components alike) can be employed as a cross-linker, making possible the cross-linking reaction at mild experimental conditions, neither requiring sophisticated lab implements nor using any potentially harmful procedure. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/28183 |
DOI: | 10.1021/bm401541v |
ISSN: | 1525-7797 |
Versão da editora: | http://pubs.acs.org/doi/pdf/10.1021/bm401541v |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17893-Biomacromolecules 2014, 15, 291−301.pdf Acesso restrito! | 1,96 MB | Adobe PDF | Ver/Abrir |