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https://hdl.handle.net/1822/2868| Título: | Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes |
| Autor(es): | Munteanu, Florentina-Daniela Ruiz-Duenas, Francisco J. T. Martinez, Angel Paulo, Artur Cavaco |
| Palavras-chave: | Bioelectrocatalysis Versatile peroxidase Graphite electrode Heterogeneous electron transfer |
| Data: | 2005 |
| Editora: | Elsevier 1 |
| Revista: | Journal of electroanalytical chemistry |
| Citação: | "Journal of electroanalytical chemistry". ISSN 0022-0728. 580:1 (June 2005) 35-40. |
| Resumo(s): | Electron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase- modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase. Rotating disk electrodes (RDE) experiments were performed at 0 V (vs. SCE) in two different buffers (tartrate buffer, pH 5.0; and citrate buffer, pH 3.0). From measurements of the mediated and mediatorless currents of H2O2 reduction at the RDE, the percentage of VP molecules involved in direct ET (≈55%) was calculated. The peroxidase-modified electrodes were used for determination of the donor substrates in RDE mode, and the results were interpreted in terms of catalytic efficiencies. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/2868 |
| DOI: | 10.1016/j.jelechem.2005.02.022 |
| ISSN: | 0022-0728 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
