Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31517
Título: | Production of extracellular L-asparaginase : from bioprospecting to the engineering of an antileukemic biopharmaceutical |
Autor(es): | Ribeiro, Artur J. Lopes, André Moreni Converti, Attilio Oliveira, Marcos Antonio de Motta, Cristina Maria de Souza Magalhães, Pérola de Oliveira Farías Avendaño, Jorge Gonzalo Mazzola, Priscila Gava Rangel-Yagui, Carlota de Oliveira Paulo, Artur Cavaco Pessoa Júnior, Adalberto |
Data: | 25-Mai-2014 |
Citação: | Ribeiro, Artur J.; Paulo, Artur Cavaco, Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical.. IPFB2014 - 8th International Conference on Polymer and Fiber Biotechnology. Braga, Portugal, May 25-27, 2014. |
Resumo(s): | The L-asparaginase (L-asparagine amino hydrolase, E.C.3.5.1.1) catalyzes the hydrolysis of L-aspargine into L-aspartic acid and ammonia. The effective depletion of L-asparine results in cytotoxicity for leukemic cell. Therefore the enzyme has been a clinically acceptable anti-tumour agent for the effective treatment of acute lymphoblastic leukemia (ALL) and lymphosarcoma. L-asparaginase production using microbial system had attracted considerable attention, owing to the cost effective and eco friendly nature. A wide range of microorganisms such as filamentous fungi, yeasts and bacteria have proved to be the good sources of the enzyme L-asparaginase. Thus, in this review mainly focuses on the biochemical aspects of L-asparaginase production, aiming to comprehend the physiochemical characteristics, such as stability, bioavailability, toxicity, allergenic aspects, application, and enzyme properties and kinetics of recombinant enzyme production by fermentation. Processes central to these biochemical aspects, including fermentation of L-asparaginase producing organisms and downstream processing of the enzyme are also discussed. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/31517 |
Versão da editora: | http://ipfb2014.wix.com/ipfb2014 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_18603_1.pdf | 95,71 kB | Adobe PDF | Ver/Abrir |