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https://hdl.handle.net/1822/33048
Título: | Use of protista and algae to study toxicity of dye compounds |
Autor(es): | Dias, Nicolina Vándrovcová, Marta Kideryová, Linda Novotný, Cenek Lima, Nelson |
Palavras-chave: | Textile dyes Toxicity bioassays Tetrahymena pyriformis Selenastrum capricornutum |
Data: | 2003 |
Editora: | Universidade do Minho. Micoteca |
Citação: | Dias, Nicolina; Vándrovcová, M.; Kideryová, L.; Novotný, C.; Lima, Nelson, Use of protista and algae to study toxicity fo dye compounds. In Nelson Lima and David Smith (Eds.), Biological Resource Centres and the Use of Microbes, Braga: Micoteca da Universidade do Minho, 2003. ISBN: 972-97916-3-5, 71-85 |
Resumo(s): | Comparative assessment of the toxicological effects of two azo dyes [Reactive Orange 16 (R016); Congo Red (CR)] and two anthraquinone dyes [Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)] was performed on two in vitro cell models, the ciliate protozoa Tetrahymena pyriformis and the microalga Selenastrum capricornutum. Growth impairment, viability assay using calcein AM and ethidium homodimer-1 (CAM/EthD-1 assay), grazing and morphometric assay were the tests performed on T pyriformis using 48 h-tests. They represented simple and fast bioassays providing overall information on the morphological and physiological state of the cells exposed to different dyes. The algal test measured growth inhibition after a 96-h exposure. The anthraquinone dye DB3 was found to be the most toxic dye among all the dyes tested. The EC50 value of 0.5 ± 0.0 mg/l detected in the algal test was 10 to 100-fold lower compared to other dyes tested. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/33048 |
ISBN: | 972-97916-3-5 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_3399_1.pdf | 664,04 kB | Adobe PDF | Ver/Abrir |