Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33098
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, Fernando | por |
dc.contributor.author | Pedrosa, Jorge | por |
dc.contributor.author | Coelho, Maria Teixeira | por |
dc.contributor.author | Guedes, Joana | por |
dc.contributor.author | Ferreirinha, Pedro | por |
dc.contributor.author | Howes, Ashleigh | por |
dc.contributor.author | Lai, Wi S. | por |
dc.contributor.author | Blackshear, Perry J. | por |
dc.contributor.author | O´Garra, Anne | por |
dc.contributor.author | Castro, António G. | por |
dc.contributor.author | Saraiva, Margarida | por |
dc.date.accessioned | 2015-01-22T14:03:59Z | - |
dc.date.available | 2015-01-22T14:03:59Z | - |
dc.date.issued | 2014-03-16 | - |
dc.identifier.issn | 0014-2980 | por |
dc.identifier.uri | https://hdl.handle.net/1822/33098 | - |
dc.description.abstract | The activation of TLRs by microbial molecules triggers intracellular-signaling cascades and the expression of cytokines such as IL-10. Il10 expression is tightly controlled to ensure effective immune responses, while preventing pathology. Maximal TLR-induction of Il10 transcription in macrophages requires signaling through the MAPKs, ERK, and p38. Signals via p38 downstream of TLR4 activation also regulate IL-10 at the post-transcriptional level, but whether this mechanism operates downstream of other TLRs is not clear. We compared the regulation of IL-10 production in TLR2 and TLR4-stimulated BM-derived macrophages and found different stability profiles for the Il10 mRNA. TLR2 signals promoted a rapid induction and degradation of Il10 mRNA, whereas TLR4 signals protected Il10 mRNA from rapid degradation, due to the activation of Toll/IL-1 receptor domain-containing adaptor inducing IFN-ß (TRIF) and enhanced p38 signaling. This differential post-transcriptional mechanism contributes to a stronger induction of IL-10 secretion via TLR4. Our study provides a molecular mechanism for the differential IL-10 production by TLR2- or TLR4-stimulated BMMs, showing that p38-induced stability is not common to all TLR-signaling pathways. This mechanism is also observed upon bacterial activation of TLR2 or TLR4 in BMMs, contributing to IL-10 modulation in these cells in an infection setting. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-Blackwell | por |
dc.rights | openAccess | por |
dc.subject | IL-10 | por |
dc.subject | MAPKs | por |
dc.subject | TLRs | por |
dc.subject | Post-transcriptional regulation | por |
dc.title | Differential post-transcriptional regulation of IL-10 by TLR2 and TLR4-activated macrophages | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/eji.201343734/full | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 856 | por |
oaire.citationEndPage | 866 | por |
oaire.citationIssue | 3 | por |
oaire.citationTitle | European Journal of Immunology | por |
oaire.citationVolume | 44 | por |
dc.date.updated | 2015-01-20T13:47:19Z | - |
dc.identifier.doi | 10.1002/eji.201343734 | por |
dc.identifier.pmid | 24227629 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | European Journal of Immunology | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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teixeira-coelho_eur j imm_2014 posp.pdf | 797,96 kB | Adobe PDF | Ver/Abrir |