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https://hdl.handle.net/1822/39315
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreirinha, Pedro | por |
dc.contributor.author | Pérez-Cabezas, Begoña | por |
dc.contributor.author | Correia, Alexandra | por |
dc.contributor.author | Miyazawa, Bruna | por |
dc.contributor.author | Carvalhais, Virgínia Maria Dinis | por |
dc.contributor.author | França, Ângela Maria Oliveira Sousa | por |
dc.contributor.author | Faustinho, Augusto | por |
dc.contributor.author | Cordeiro-da-Silva, Anabela | por |
dc.contributor.author | Teixeira, Luzia | por |
dc.contributor.author | Pier, Geral | por |
dc.contributor.author | Cerca, Nuno | por |
dc.contributor.author | Vilanova, Manuel | por |
dc.date.accessioned | 2016-01-08T16:27:04Z | - |
dc.date.available | 2016-01-08T16:27:04Z | - |
dc.date.issued | 2015-09-22 | - |
dc.identifier.citation | Ferreirinha, P.; Pérez-Cabezas, B.; Correia, A.; Miyazawa, B.; Carvalhais, V.; França, Ângela; Faustinho, A.; Cordeiro-da-Silva, A.; Teixeira, L.; Pier, G. B.; Cerca N; Vilanova, M., Poly-N-acetylglucosamine expression in Staphyloccocus epidermidis biofilm cells affects the imune response and promotes tissue pathology in infected hosts. XXXVI Annual Meeting of the Portuguese Society for Immunology. Braga, Portugal, 22-24 September, 2015. | - |
dc.identifier.uri | https://hdl.handle.net/1822/39315 | - |
dc.description.abstract | Staphylococcus epidermidis is a biofilm - forming bacterium and a leading etiological agent of nosocomial infections. The ability to establish biofilms on indwelling medical devices is a key virulence factor for this bacterium. Still, the influence of poly - N - acetyl glucosamine (PNAG), the major component of the extracellular biofilm matrix, in the host immune response has been scarcely studied. Here, t h is influence was assessed in mice challenged i.p. with PNAG - p roducing (WT) and isogenic - mutant lacking PNAG (M10) bacteria grown in biofilm - inducing conditions. Faster bacterial clearance was observed in the mice infected with WT bacteria than in M10 - infected counterparts , which w as accompanied by earlier neutrophil recruitment and higher IL - 6 production. Interestingly, in the WT - infected mice, but not in those infected with M10 , elevated serum IL - 10 was detected . To further study the effe ct of PNAG in the immune response, mice were primed with WT or M10 biofilm bacteria and subsequently infected with WT biofilm - released cells. WT - primed mice presented a higher frequency of splenic IFN - γ + and IL - 17 + CD4 + T cells, and more severe liver patho logy than M10 - primed counterparts. Nevertheless, T reg cells obtained from the WT - primed mice presented a higher suppressive function than those obtained from M10 - primed mice. This effect was abrogated when IL - 10 - deficient mice were similarly primed and infected indicating that PNAG promotes the differentiati on of highly suppressive T reg cells by a mechanism dependent on IL - 10. Altogether, these results provide evidence help ing explain ing the coexistence of inflammation and bacterial persistence often observed in biofilm - originated S. epidermidis infections | por |
dc.description.sponsorship | FCT and COMPETE grants PTDC/BIA-MIC/113450/2009 and FCOMP-01-0124-FEDER-14309. PF, AC, LT and VC were supported by FCT grants SFRH/BD/76900/2011, SFRH/BPD/91623/2012, IF/01241/2014, SFRH/BD/78235/2011 | por |
dc.language.iso | eng | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/113450/PT | - |
dc.relation | SFRH/BD/76900/2011 | - |
dc.relation | SFRH/BPD/91623/2012 | - |
dc.relation | SFRH/BD/78235/2011 | - |
dc.rights | restrictedAccess | por |
dc.title | Poly-N-acetyl glucosamine expression in Staphylococcus epidermidis biofilm cells affects the immune response and promotes tissue pathology in infected hosts | por |
dc.type | conferenceAbstract | - |
dc.peerreviewed | yes | por |
dc.comments | CEB26575 | por |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | 20 Set. - 22 Set. 2015 | por |
sdum.event.type | conference | por |
oaire.citationConferencePlace | Braga, Portugal | por |
oaire.citationTitle | XXXVI Annual Meeting of the Portuguese Society for Immunology | por |
dc.date.updated | 2015-11-27T14:18:31Z | - |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
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