Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/3941
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Endres, M. | - |
dc.contributor.author | Hutmacher, D. W. | - |
dc.contributor.author | Salgado, A. J. | - |
dc.contributor.author | Kaps, C. | - |
dc.contributor.author | Ringe, J. | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Sittinger, M. | - |
dc.contributor.author | Brandwood, A. | - |
dc.contributor.author | Schantz, J. T. | - |
dc.date.accessioned | 2006-01-16T16:17:49Z | - |
dc.date.available | 2006-01-16T16:17:49Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | "Tissue Engineering". ISSN 1076-3279. 9:4 (Aug. 2003) 689-702. | eng |
dc.identifier.issn | 1076-3279 | - |
dc.identifier.uri | https://hdl.handle.net/1822/3941 | - |
dc.description.abstract | The aim of this project was to investigate the in vitro osteogenic potential of human mesenchymal progenitor cells in novel matrix architectures built by means of a three-dimensional bioresorbable synthetic framework in combination with a hydrogel. Human mesenchymal progenitor cells (hMPCs) were isolated from a human bone marrow aspirate by gradient centrifugation. Before in vitro engineering of scaffold-hMPC constructs, the adipogenic and osteogenic differentiation potential was demonstrated by staining of neutral lipids and induction of bone-specific proteins, respectively. After expansion in monolayer cultures, the cells were enzymatically detached and then seeded in combination with a hydrogel into polycaprolactone (PCL) and polycaprolactone-hydroxyapatite (PCL-HA) frameworks. This scaffold design concept is characterized by novel matrix architecture, good mechanical properties, and slow degradation kinetics of the framework and a biomimetic milieu for cell delivery and proliferation. To induce osteogenic differentiation, the specimens were cultured in an osteogenic cell culture medium and were maintained in vitro for 6 weeks. Cellular distribution and viability within three-dimensional hMPC bone grafts were documented by scanning electron microscopy, cell metabolism assays, and confocal laser microscopy. Secretion of the osteogenic marker molecules type I procollagen and osteocalcin was analyzed by semiquantitative immunocytochemistry assays. Alkaline phosphatase activity was visualized by p-nitrophenyl phosphate substrate reaction. During osteogenic stimulation, hMPCs proliferated toward and onto the PCL and PCL-HA scaffold surfaces and metabolic activity increased, reaching a plateau by day 15. The temporal pattern of bone-related marker molecules produced by in vitro tissue-engineered scaffold-cell constructs revealed that hMPCs differentiated better within the biomimetic matrix architecture along the osteogenic lineage. | eng |
dc.description.sponsorship | National University of Singapore Young Investigator Award WBS No. R-397-000-003-650. | por |
dc.language.iso | eng | eng |
dc.publisher | Mary Ann Liebert | eng |
dc.rights | openAccess | eng |
dc.title | Osteogenic induction of human bone marrow-derived mesenchymal progenitor cells in novel synthetic polymer-hydrogel matrices | eng |
dc.type | article | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 689 | por |
oaire.citationEndPage | 702 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 9 | por |
dc.identifier.doi | 10.1089/107632703768247386 | por |
dc.identifier.pmid | 13678447 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Tissue Engineering | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |