Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40913
Título: | Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
Autor(es): | Correia, Clara R. Pirraco, Rogério P. Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Mano, J. F. |
Palavras-chave: | Bioinspired materials Biomedical engineering Biomineralization Growth factor signalling Stem-cell research |
Data: | 2016 |
Editora: | Nature Publishing Group |
Revista: | Scientific Reports |
Citação: | Correia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016 |
Resumo(s): | A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/40913 |
DOI: | 10.1038/srep21883 |
ISSN: | 2045-2322 |
Versão da editora: | http://www.nature.com/articles/srep21883 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18675-Artigo.pdf | 1,63 MB | Adobe PDF | Ver/Abrir |