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https://hdl.handle.net/1822/44547
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, E. | por |
dc.contributor.author | Lopes, Susana Patrícia | por |
dc.contributor.author | Pereira, C. | por |
dc.contributor.author | Azevedo, Nuno Filipe | por |
dc.contributor.author | Lourenço, Anália | por |
dc.contributor.author | Henriques, Mariana | por |
dc.contributor.author | Pereira, Maria Olívia | por |
dc.date.accessioned | 2017-02-01T11:24:22Z | - |
dc.date.available | 2017-02-01T11:24:22Z | - |
dc.date.issued | 2017-01-23 | - |
dc.identifier.citation | Rodrigues, E.; Lopes, Susana P.; Pereira, C.; Azevedo, Nuno Filipe; Lourenço, Anália; Henriques, Mariana; Pereira, Maria Olívia, Polymicrobial ventilator-associated pneumonia: fighting in vitro Candida albicans-Pseudomonas aeruginosa biofilms with antifungal-antibacterial combination therapy. PLoS One, 12(1), e0170433, 2017 | por |
dc.identifier.issn | 1932-6203 | por |
dc.identifier.uri | https://hdl.handle.net/1822/44547 | - |
dc.description.abstract | The polymicrobial nature of ventilator-associated pneumonia (VAP) is now evident, with mixed bacterial-fungal biofilms colonizing the VAP endotracheal tube (ETT) surface. The microbial interplay within this infection may contribute for enhanced pathogenesis and exert impact towards antimicrobial therapy. Consequently, the high mortality/morbidity rates associated to VAP and the worldwide increase in antibiotic resistance has promoted the search for novel therapeutic strategies to fight VAP polymicrobial infections. Under this scope, this work aimed to assess the activity of mono- vs combinational antimicrobial therapy using one antibiotic (Polymyxin B; PolyB) and one antifungal (Amphotericin B; AmB) agent against polymicrobial biofilms of Pseudomonas aeruginosa and Candida albicans. The action of isolated antimicrobials was firstly evaluated in single- and polymicrobial cultures, with AmB being more effective against C. albicans and PolyB against P. aeruginosa. Mixed planktonic cultures required equal or higher antimicrobial concentrations. In biofilms, only PolyB at relatively high concentrations could reduce P. aeruginosa in both monospecies and polymicrobial populations, with C. albicans displaying only punctual disturbances. PolyB and AmB exhibited a synergistic effect against P. aeruginosa and C. albicans mixed planktonic cultures, but only high doses (256 mg L-1) of PolyB were able to eradicate polymicrobial biofilms, with P. aeruginosa showing loss of cultivability (but not viability) at 2 h post-treatment, whilst C. albicans only started to be inhibited after 14 h. In conclusion, combination therapy involving an antibiotic and an antifungal agent holds an attractive therapeutic option to treat severe bacterial-fungal polymicrobial infections. Nevertheless, optimization of antimicrobial doses and further clinical pharmacokinetics/pharmacodynamics and toxicodynamics studies underpinning the optimal use of these drugs are urgently required to improve therapy effectiveness and avoid reinfection. | por |
dc.description.sponsorship | Support was provided by: ESCMID Research Grant 2014, named "Reconstruction of polymicrobial interactions in infections: the case of Pseudomonas aeruginosa and Candida albicans cross talk in ventilator-associated pneumonia" and funded by European Society of Clinical Microbiology and Infectious Diseases (ESCMID) [https://www.escmid.org/profession_career/ awards_grants/research_grants/previous_ awardees/awardees_2014/] (to AL); Portuguese Foundation for Science and Technology (FCT), COMPETE2020, FEDER and P2020, under the scope of the strategic funding of UlD/B10/04469/2013 (URL: http://www.ceb.uminho.pt/Projects/Details/2590) (CEB, UMinho) and UID/EQU/00511/2013 units. (URL:https://sigarra.up.ptispup/pt/ noticias_geral.ver_noticia?p_nr=17725) (LEPABE, FEUP); Project POCI-01-0145-FEDER-006684 (URL: http://www.ceb.uminho.pt/Projects/Details/ 2590) (CEB, Uminho) and POCI-01-0145-FEDER-006939 (URL: https://sigarra.up.pt/feup/pt/ noticias_geral.ver_noticia?p_nr=50509) (LEPABE, FEUP), financed by COMPETE2020, FEDER and National Funds; FCT and the European Community fund FEDER, through Program COMPETE, under the scope of project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) (URL: http// www.ceb.uminho.pt/Projects/Details/1505) (Manuel Mota, CEB, Uminho); and Post-doc grants of the author SPL (SFRH/BPD/95616/2013) and MER (SFRH/BPD/95401/2013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.The authors would like to acknowledge the ESCMID Research Grant 2014, funded by European Society of Clinical Microbiology and Infectious Diseases (ESCMID). They also thanks to the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UID/BIO/04469/2013 and UID/EQU/00511/2013 units and COMPETE 2020 (POCI-01-0145-FEDER-006684 and POCI-01-0145-FEDER-006939), and by FCT and the European Community fund FEDER, through Program COMPETE, under the scope of project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). T | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.rights | openAccess | por |
dc.subject | Mixed biofilm | por |
dc.subject | Candida albicans | por |
dc.subject | Pseudomonas aeruginosa | por |
dc.subject | Polymicrobial infection | por |
dc.subject | Ventilator-associated pneumonia | por |
dc.subject | Combinational antimicrobial therapy | por |
dc.title | Polymicrobial ventilator-associated pneumonia : fighting in vitro Candida albicans-Pseudomonas aeruginosa biofilms with antifungal-antibacterial combination therapy | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://journals.plos.org/plosone/ | por |
dc.comments | CEB46570 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 19 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | PLoS ONE | por |
oaire.citationVolume | 12 | por |
dc.date.updated | 2017-01-29T15:05:24Z | - |
dc.identifier.eissn | 1932-6203 | por |
dc.identifier.doi | 10.1371/journal.pone.0170433 | por |
dc.identifier.pmid | 28114348 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | PLoS ONE | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
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document_46570_1.pdf | 1,86 MB | Adobe PDF | Ver/Abrir |