Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/46995
Título: | Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate |
Autor(es): | Silva, Joana M. García, José R. Reis, R. L. García, Andrés J. Mano, J. F. |
Palavras-chave: | Cell adhesion Cross-linking Fibronectin Surface chemistry Tissue engineering |
Data: | Jan-2017 |
Editora: | Elsevier Ltd. |
Revista: | Acta Biomaterialia |
Citação: | Silva J. M., Garcia J., Reis R. L., Garcia A. J., Mano J. F. Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate, Acta Biomaterialia, Vol. n/d, doi:10.1016/j.actbio.2017.01.058, 2017 |
Resumo(s): | Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multilayers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical and chemical properties of these films, enabling the transition from cell-adhesive to cellresistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46995 |
DOI: | 10.1016/j.actbio.2017.01.058 |
ISSN: | 1742-7061 |
e-ISSN: | 1878-7568 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S174270611730065X |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19010-1-s2.0-S174270611730065X-main.pdf Acesso restrito! | 4,55 MB | Adobe PDF | Ver/Abrir |