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https://hdl.handle.net/1822/47657
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Campo DC | Valor | Idioma |
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dc.contributor.author | Endersen, Lorraine | por |
dc.contributor.author | Buttimer, Colin | por |
dc.contributor.author | Nevin, Eoghan | por |
dc.contributor.author | Coffey, Aidan | por |
dc.contributor.author | Neve, Horst | por |
dc.contributor.author | Oliveira, Hugo Alexandre Mendes de | por |
dc.contributor.author | Lavigne, Rob | por |
dc.contributor.author | O'Mahony, Jim | por |
dc.date.accessioned | 2017-11-22T14:31:23Z | - |
dc.date.available | 2017-11-22T14:31:23Z | - |
dc.date.issued | 2017-07-17 | - |
dc.identifier.citation | Endersen, Lorraine; Buttimer, Colin; Nevin, Eoghan; Coffey, Aidan; Neve, Horst; Oliveira, Hugo; Lavigne, Rob; O'Mahony, Jim, Investigating the biocontrol and anti-biofilm potential of a three phage cocktail against Cronobacter sakazakii in different brands of infant formula. International Journal of Food Microbiology, 253, 1-11, 2017 | por |
dc.identifier.issn | 0168-1605 | por |
dc.identifier.uri | https://hdl.handle.net/1822/47657 | - |
dc.description | Supplementary data to this article can be found online at: http://dx. doi.org/10.1016/j.ijfoodmicro.2017.04.009. | por |
dc.description.abstract | In recent years, the microbiological safety of powdered infant formula has gained increasing attention due to the identification of contaminating C. sakazakii and its epidemiological link with life-threatening neonatal infections. Current intervention strategies have fallen short of ensuring the production of infant formula that is free from C. sakazakii. In this study, we describe the isolation and characterisation of three bacteriophages (phages) and their application as a phage cocktail to inhibit the growth of C. sakazakii in different brands of infant formula, while also assessing the phages ability to prevent biofilm formation. All three phages, isolated from slurry, possess a relatively broad host range, verified by their ability to infect across genera and species. When all three phages were combined and used as part of a phage cocktail, 73% coverage was obtained across all Cronobacter strains tested. Optimum thermo-tolerance and pH stability were determined between 4 °C37 °C, and pH 68, respectively, well within the normal range of application of infant formula. Genome sequencing and analysis revealed all the phages to be free from lysogenic properties, a trait which renders each favourable for phage therapy applications. As such, the combined-phage preparation (3 × 108 pfu/mL) was found to possess a strong bactericidal effect on C. sakazakii/C. sakazakii LUX cells ( 104 cfu/mL), resulting in a significant reduction in cell numbers, to below the limit of detection (< 10 cfu/mL). This was observed following a 20 h challenge in different brands of infant formula, where samples in the absence of the phage cocktail reached concentrations of ~ 109 cfu/mL. The phage cocktail also demonstrated promise in preventing the establishment of biofilm, as biofilm formation could not be detected for up to 48 h post treatment. These results highlight the potential application of this phage preparation for biocontrol of C. sakazakii contamination in reconstituted infant formula and also as a preventative agent against biofilm formation. | por |
dc.description.sponsorship | This work was funded by Technological Sector Research Strand III ref. CRS/07/CR03. Angela Back from MRI Kiel is acknowledged for technical assistance in preparations for electron microscopy. Hugo Oliveira and Rob Lavigne contributed to the genome sequencing analysis, supported by the KULeuven GOA (GOA/15/006) Grant Phagebiosystems. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Bacteriophages | por |
dc.subject | Biocontrol | por |
dc.subject | Biofilm | por |
dc.subject | Cronobacter sakazakii | por |
dc.subject | Infant formula | por |
dc.subject | Biofihn | por |
dc.title | Investigating the biocontrol and anti-biofilm potential of a three phage cocktail against Cronobacter sakazakii in different brands of infant formula | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/international-journal-of-food-microbiology/ | por |
dc.comments | CEB46763 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 11 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 253 | por |
dc.date.updated | 2017-08-03T11:19:45Z | - |
dc.identifier.eissn | 0168-1605 | por |
dc.identifier.doi | 10.1016/j.ijfoodmicro.2017.04.009 | por |
dc.identifier.pmid | 28460269 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Food Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46763_1.pdf | 1,91 MB | Adobe PDF | Ver/Abrir |